Potential Role of Sirtuins As Epigenetic Biomarkers of the Patient with Atherosclerosis or Calcific Aortic Stenosis

IRCCS SYNLAB SDN3 enrolled

Overview

The project aims to shed light on the role of Sirtuins, enzymes belonging to the third class of histone deacetylases (class III) involved in epigenetic modifications (deacetylation), focusing mainly on Sirtuin 6 (SIRT6), as a regulator of atherosclerosis and severe aortic valve stenosis through molecular-based and epigenetic studies in human VSMC cells.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Atherosclerosis and Calcific Aortic Stenosis with and Without Diabetes

Interventions

lncRNA signature analysesDIAGNOSTIC_TEST

Specific validation experiments will be performed by Real-Time PCR (C-reactive protein), Western Blot, flow cytometry and microscopy

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Patients with atherosclerosis or calcific aortic stenosis with and without diabetes older than 18 years of either sex; * Patients without atherosclerosis undergoing surgical procedure for endocarditis; * Patients who will have signed informed consent Exclusion Criteria: * Patients for whom biological material cannot be recovered for study purposes; * Patients who refuse to participate in the study

Locations (1)

Irccs Synlab Sdn

Naples, Italy

RECRUITING

Outcomes

Primary Outcomes

To evaluate by analysis of data obtained from NGS techniques whether and which proteins belonging to the Sirtuin family may be deregulated in patients with atherosclerosis or calcific aortic stenosis

Time frame: 36 months

Secondary Outcomes

To evaluate the experimental results achieved in the clinical-diagnostic context

Time frame: 36 months

Data from ClinicalTrials.gov

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