Sleep is an important modulator of the immune response, whereby sleep disturbances (ie, poor sleep quality, insufficient sleep and/or primary sleep disorder, obstructive sleep apnea (OSA)) contribute to inflammatory disease risk and dysregulation of immune response in front of infectious agents. The objective of this study is to evaluate the impact of undiagnosed and non-treated sleep disorders on innate immunity in a cohort of COVID-19 patients and the role of trained immunity induced by influenza vaccination in the innate immune response.
Sleep and immune system have reciprocal relationship. Sleep has a restorative role on immune system, influencing innate and adaptive immunity and sleep disorders can decrease immune response. Healthy innate immunity is crucial into regulation of the response against SARS-CoV-2. The hypothesis of the study is that the innate immunity response is blunted by sleep disorders and, this mitigated immune response, could influence on COVID-19 severity. Impaired immune response in patients with sleep disorders could be ameliorated inducing trained immunity by influenza vaccine.
Study Type
OBSERVATIONAL
Enrollment
50
All participants will take influenza vaccine (Influvac Tetra, Abbott Biologicals, IL, USA) and the trained immune response will be evaluated.
Hospital Universitari Parc Tauli
Sabadell, Barcelona, Spain
RECRUITINGImmunological study: cytokines.
To measure in blood: IL-1 beta, IL-6, IL-8, IL-10, TNF-alfa, IFN-alfa, IFN-gamma, GM-CSF.
Time frame: To evaluate immune response blood samples will be taken at the time influenza vaccine is given, 7 and 30 days later.
Innate cells: monocytes, classical dendritic cells and Natural Killer cells.
To measure: monocytes (CD45, CD14, CD16, HLA-DR), classical dendritic cells (CD45, CD1c, CD141, CD11c, CD86) and NK cells (CD45, CD56, CD16, Pan-KIR2D, NKG2D
Time frame: To evaluate immune response blood samples will be taken at the time influenza vaccine is given, 7 and 30 days later.
Epigenetic modification.
To measure: H3K4me3, H3K4me1 and H3K27Ac
Time frame: To evaluate immune response blood samples will be taken at the time influenza vaccine is given, 7 and 30 days later.
Diagnostic of obstructive sleep apnea in selected cohort
Night home sleep study with WatchPAT® 300.
Time frame: It is an overnight study that will last for one night.
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.