Investigation of Acupoint in Irritable Bowel Syndrome Based on Biological Characteristics

Inclusion Criteria: * Inclusion criteria for healthy volunteers: 1. 18 years old ≤ age ≤75 years old, gender is not limited; 2. No inflammatory bowel disease, lactose intolerance, intestinal bacterial overgrowth, parasitic infection, drug induced enteritis and other intestinal diseases; 3. No abdominal pain, frequency of defecation and changes in fecal characteristics in 1 week; 4. Provide healthy subjects with no obvious abnormalities reported by colonoscopy in the past 1 year; 5. Healthy subjects who can provide medical examination reports within the past 1 year and have routine physical examination conducted by researchers to confirm that there are no heart and lung diseases, digestive, urinary, blood, endocrine, nervous system and other serious underlying diseases; 6. No antidiarrheal drugs, antibiotics, gastrointestinal motility drugs or other related drugs affecting clinical studies within 4 weeks; 7. Clear consciousness, can complete normal communication; 8. Understands and is willing to comply with the research protocol and sign the informed consent. * Inclusion criteria for IBS patients: 1. Patients who can provide colonoscopy report in the past 1 year and meet the Rome IV standard of irritable bowel syndrome and TCM syndrome differentiation type; 2. Provide medical examination reports within the past 1 year to confirm patients without digestive, circulatory, respiratory, urinary, reproductive, endocrine, nervous system and other malignant tumors and serious underlying diseases; 3. 18 years old ≤ age ≤75 years old, gender is not limited; 4. No antidiarrheal drugs, antibiotics, gastrointestinal motility drugs or other related drugs that affect clinical studies have been taken within 4 weeks; 5. Clear consciousness, can complete normal communication; 6. Understands and is willing to comply with the research protocol and sign the informed consent. Exclusion Criteria: * Exclusion criteria for healthy volunteers: 1. Those who have mental illness, alcohol dependence or a history of drug abuse and cognitive impairment are unable to cooperate with the examination and understanding of the subject; 2. Female volunteers who are pregnant or lactating, or in ovulation and menstruation period; 3. Volunteers with current abnormal body temperature; 4. Subjects currently participating in other clinical trials. * Exclusion criteria for patients with IBS: 1. Those who have mental illness, alcohol dependence or a history of drug abuse and cognitive impairment are unable to cooperate with the examination and understanding of the subject; 2. Pregnant or lactating women or female subjects in ovulation and menstruation period; 3. Patients with inflammatory bowel disease, lactose intolerance, intestinal bacterial overgrowth, parasitic infections, drug-induced enteritis and other intestinal diseases; 4. Patients with intestinal diseases such as functional constipation, functional diarrhea, opioid-induced constipation, and non-specific functional bowel disease; 5. Patients with functional gastrointestinal diseases such as functional dyspepsia, functional heartburn, central abdominal pain syndrome; 6. Patients with current abnormal body temperature; 7. Participants who are currently participating in other clinical trials.