The study aimed to check the 2000m ergometer test on markers of gut permeability in the competitive phase of rowers. 18 members of the Polish rowing team took part in the study.
The phenomenon of epithelial wall leakage has been the subject of investigations within athletic domains such as running and cycling. Nevertheless, there exists an insufficiency of understanding regarding gut permeability in alternative disciplines, such as rowing, particularly following the completion of a standard competitive distance of 2000 meters. Hence, the principal objective of our study was to assess gut permeability after completing a 2000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 2000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1-hour of restitution. Parameters, such as I-FABP, LPS, LBP, and zonulin, were determined using appropriate biochemical tests.
Study Type
OBSERVATIONAL
Enrollment
18
2000meter rowing ergometer test
Poznan University of Physical Education
Poznan, Poland
I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury
I-FABP concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
zonulin to measure tight junction leakage
zonulin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LBP (lipopolysaccharide binding protein) to measure endotoxin
LBP concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LPS (lipopolysaccharide) to measure endotoxin
LPS concentration measured in blood \[EU/Ll\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Lactic acid to measure fatigue after the race
Lactate concentration measured in capillary blood from earlobe \[mmol/L\] using a commercially available kit (Diaglobal, Berlin, Germany)
Time frame: Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
energy
energy \[kcal \]of food intake measured by 24h dietary recall
Time frame: day before test
fiber intake
fiber \[g\] intake day before the test measured by 24h dietary recall
Time frame: day before test
protein
protein \[g\] intake day before the test measured by 24h dietary recall
Time frame: day before test
carbohydrate
carbohydrate \[g\] intake day before the test measured by 24h dietary recall
Time frame: day before test
body mass
Body mass \[kg\] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Time frame: in the morning before the test
Body composition - water
% of body water measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Time frame: in the morning before the test
Body composition - fat
% of body fat measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Time frame: in the morning before the test
Lean body mass
Lean body mass \[kg\] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Time frame: in the morning before the test