Effect of Dupilumab on Nasal Epithelial Barrier Function in Patients With CRSwNP

Medical University of Vienna80 enrolled

Overview

The investigators aim to investigate two major so far unresolved topics in CRSwNP research: (1) Thorough functional and molecular characterisation of barrier function in patients suffering from CRSsNP and CRSwNP and (2) effect of dupilumab treatment on barrier function in polyp patients. This will be achieved in patient-derived samples by employing measurement of barrier function in primary cell cultures in combination with a mass cytometry based imaging approach, transcriptomic analysis as well as cytokine and microbiome data of individual patients.

Primary objective 1: Characterization of differences in barrier function of the nasal epithelium in patients suffering from CRSsNP, CRSwNP with and without asthma or N-ERD (in absence of therapy with monoclonal antibodies). T Primary objective 2: Effect of dupilumab treatment on barrier function of the nasal epithelium in patients suffering from CRSwNP with and without asthma or N-ERD.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Chronic Rhinosinusitis With Nasal Polyps Chronic Rhinosinusitis Without Nasal Polyps

Interventions

Eligibility

Sex: ALLMin age: 18 YearsMax age: 99 Years

Medical Language ↔ Plain English

Inclusion Criteria: * 18-99 years of age * Willingness to participate in the study * Suffer from chronic rhinosinusitis defined as in EPOS 2020 criteria, see main text * Group 1 (n=20, CRSsNP): Absence of nasal polyps * Group 2 (N=60, CRSwNP): Presence of nasal polyps as confirmed by endoscopy or CT and planned therapy with dupilumab * Presence or absence of non-steroidal anti-inflammatory drug (NSAID)-Exacerbated Respiratory Disease (N-ERD) * Patients with a history of treatment with monoclonal antibodies will only be included if at least a washout period of 6 months has passed Exclusion Criteria: * Pregnancy (as determined by ß-HCG test) or breast feeding * Patients with severe anatomic variations or deviations that do not allow access to all areas in the nasal cavity * Patients with cystic fibrosis or primary ciliary dyskinesia * Patients with permanent immunosuppression * A mental condition rendering the subject unable to understand the nature, scope, and possible consequences of the study * Patients with clinically meaningful comorbidity as determined by the evaluating committee * Patients with a history of exacerbation of chronic rhinosinusitis 4 weeks prior to the screening visit * Intake of a burst of systemic corticosteroids for the treatment of CRS 4 weeks prior to the screening visit

Outcomes

Primary Outcomes

Barrier function in CRSsNP and CRSwNP

Cell index (xCELLigence)

Time frame: 1 Visit (baseline)

Barrier function in CRSwNP with Dupilumab