Age Related Differences in Respiratory Immune Responses in Influenza Virus Infection

N/AEnrolling By InvitationNCT06401720

Karolinska Institutet800 enrolled

Overview

The goal of this observational study is to understand immune responses to viral airway infection in adults, including the elderly. The main question(s) to answer is/are: Why do some individuals acquire only asymptomatic or mild Influenza A virus (IAV) infection while others become severely ill and even succumb to the same disease? Participants will be asked to donate samples when seeking health care for influenza-like symptoms or if hospitalized for IAV or SARS-CoV-2. Samples asked for are: * Blood sample by venepuncture * Blood sample by capillary sampling * Nasopharyngeal aspirate * Nasopharyngeal swab * Endotracheal tube aspirate * Nasal swab * Nasal curette * Breath Explor (sampling of expired air) Researchers will compare obtained results with the same type of samples from healthy controls.

This observational study will specifically investigate different immunological parameters in blood and at the site of infection in patients with IAV symptoms or confirmed infection to understand parameters related to disease course. Blood and airway samples will be analyzed for immune cell subsets and their functionality, antibodies and other soluble mediators and linked to clinical data. Immune cells will be analyzed using multi-color flow cytometry, RNA sequencing, and in vitro functionality assays. Fluids (plasma, serum, supernatants from airway samples and cell culture supernatants) will be analyzed using ELISA (and or similar methods), proximity extension assay (Olink), etc.

Study Type

OBSERVATIONAL

Enrollment

800

Conditions

Influenza A Infection Influenza B Virus Infection SARS CoV 2 Infection

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria Patients: * Influenza A like symptoms and/or * Confirmed airway infection Exclusion Criteria Patients: * Current malignancies * Immunosuppressive treatment, not including/except hydrocortisone Exclusion Criteria Controls: * Airway infection within the past four weeks * Ongoing antibiotic treatment * Immunosuppressive treatment

Locations (5)

Södertälje Sjukhus

Södertälje, Sweden

Familjeläkarna

Stockholm, Sweden

Haga Närakut

Stockholm, Sweden

Karolinska Universitetssjukhuset

Stockholm, Sweden

Sabbatsbergs sjukhus

Stockholm, Sweden

Outcomes

Primary Outcomes

Frequency of immune cells

Flow cytometric analysis of white blood cell phenotype and frequency in PBMC, nasopharyngeal aspirate, nasopharyngeal swab, nasal swab, nasal curette, and endotracheal tube aspirate samples.

Time frame: Samples are taken at several different occasions when the patient is experiencing an airway infection and during convalescence. Samples are taken during a time frame of up to 6 months.