The aim of this clinical trial is to investigate the longitudinal effects of hydrolyzed collagen ingestion combined with resistance training on muscle-tendon unit structure and function in middle-aged males and females. The main research questions this clinical trial aims to answer are: 1. Does resistance training with hydrolyzed collagen ingestion lead to greater changes in tendon properties than resistance training alone in middle-aged health men and women? 2. Does resistance training with hydrolyzed collagen ingestion lead to greater changes in muscle size than resistance training alone? 3. Does resistance training with hydrolyzed collagen lead to greater increases in strength and power compared to resistance training alone in middle-aged, healthy men and women? Participants will be randomly assigned to collagen or placebo groups. Participants will perform 24 sessions of high intensity resistance training across 12-weeks. Alongside each training session, participants will consume a beverage containing hydrolyzed collagen or maltodextrin, with both beverages containing vitamin C. Researchers will compare the collagen and placebo groups to see if there would be beneficial effects on changes in muscle and tendon that are greater than resistance training alone. To achieve this, an dynamometry will be used to assess lower limb strength and ultrasound will be used to measure the morphological, mechanical, and material properties of the patellar tendon, as well the size and architecture of the vastus lateralis muscle.
Healthy, active, middle-aged men and women will ingest a beverage containing 30 g of hydrolyzed collagen with 50 mg of vitamin C or a calorie matched beverage (maltodextrin), also with 50 mg of vitamin C combined with high-volume, high-intensity resistance training for the lower body on 2 - 3 occasions per week for 8-12 weeks. The aim of this study was to investigate the effect of combining hydrolyzed collagen with resistance training in middle-aged men and women.If supplementation with hydrolyzed collagen leads to a greater change in tendon size, stiffness, and Young's modulus than resistance training alone, this will allow us to recommend this type of intervention to young athletes seeking to improve tendon health and/or athletic performance. This is the first study to investigate the combination of hydrolyzed collage supplementation with resistance training in middle-aged men and women. If supplementation with hydrolyzed leads to greater improvements in tendon size, stiffness, strength and/or power, this will allow us to recommend this type of intervention to middle-aged athletes/trainees seeking to improve tendon health, reduce injury risk, or enhance athletic performance. The experimental design of both arms will be the same, however Arm 1 will be health, middle-aged male participants and Arm 2 will be health, middle-aged female participants.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
TRIPLE
Enrollment
42
Participants performed 24 lower limb resistance training sessions over under supervision from the principal investigator. Participants were pair-matched, then allocated to hydrolysed collagen (COL) or a calorie-matched beverage comprising maltodextrin and non-caloric sweetener (PLA). Both COL and PLA consumed their respective supplements in conjunction with each resistance training session. Baseline assessments of strength were used to set initial training loads. An example of weekly training is included below for indicative purposes: Week 1 protocol: Day 1: * Barbell squat, 4 sets @ 90% 10-RM * Romanian deadlift, 4 sets @ 90% 10-RM Day 2: * Hex bar deadlift, 4 sets @ 90% 10-RM * Dumbbell goblet squat, 4 sets @ 90% 10-RM Linear progression adjusted weekly loads. If participants completed the prescribed sets and reps, loads increased by 2.5-5 % the following week.
Dr Rob Erskine
Liverpool, United Kingdom
m. vastus lateralis thickness (in millimetres)
Ultrasonography was used to determine changes in muscle thicknessprotocols
Time frame: 8-12 weeks' training and nutrition intervention
m. vastus lateralis fascicle pennation angle (in degrees)
Ultrasonography was used to determine the pennation angle of fascicles as they insert into the aponeurosis
Time frame: 8-12 weeks' training and nutrition intervention
Patellar tendon cross sectional area (in squared millimetres) at 3 regions along the tendon length
Ultrasonography was used to determine changes in tendon cross sectional area at 25, 50, and 75 % of tendon length
Time frame: 8-12 weeks' training and nutrition intervention
Patellar tendon stiffness (in Newtons per millimetre)
Synchronized ultrasonography, dynamometry, and surface electromyography were used to determine changes in patellar tendon stiffness
Time frame: 8-12 weeks' training and nutrition intervention
Bilateral vertical countermovement jump height (in centimetres)
Changes in jump height were estimated by use of the Optojump system (Microgate, Bolzano, Italy) and force plates (Force Decks Dual Force Plate System, VALD, Charlotte NC, USA).
Time frame: 8-12 weeks' training and nutrition intervention
Knee extensor maximal isometric torque (in Newton metres)
Changes in maximal knee extension strength were measured using isometric dynamometry (Biodex System 3, IPRS Mediquipe Limited, Lancashire, UK)
Time frame: 8-12 weeks' training and nutrition intervention
Knee extensor rate of torque development (in Newton metres per second)
Changes in explosive knee extension strength were measured using isometric dynamometry (Biodex System 3, IPRS Mediquipe Limited, Lancashire, UK)
Time frame: 8-12 weeks' training and nutrition intervention
Barbell back squat 10-repetition maximum (in kilograms)
Changes in dynamic lower body strength were measured using the maximum load during 10 repetitions of the barbell back squat exercise with standardised technique
Time frame: 8-12 weeks' training and nutrition intervention
Bilateral horizontal broad jump distance (in centimetres)
Changes in maximal horizontal displacement were measured as the maximum distance covered during a broad jump on standardised surface without footwear
Time frame: 8-12 weeks' training and nutrition intervention
Lower limb maximal isometric force (in Newtons)
Force plates (Force Decks Dual Force Plate System, VALD, Charlotte NC, USA) were used to measure changes in maximal multi-joint isometric strength during the mid-thigh pull exercise
Time frame: 8-12 weeks' training and nutrition intervention
Lower limb isometric rate of force development (in Newtons per second)
Force plates (Force Decks Dual Force Plate System, VALD, Charlotte NC, USA) were used to measure changes in explosive multi-joint isometric strength during the mid-thigh pull exercise
Time frame: 8-12 weeks' training and nutrition intervention
20-metre sprint time (in seconds)
Double photocell timing gates (Witty System, Microgate SRL, Bolzano, Italy) were used to measure changes in time taken to cover 20-metres during an all out sprint on a standardised surface
Time frame: 8-12 weeks' training and nutrition intervention
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