The Association Between Food Timing and Composition on Gastrointestinal Injury, Stress Hormone and Gastrointestinal Complains.

Poznan University of Physical Education13 enrolled

Overview

The study aimed to check the influence of timing and composition of diet on markers of gut injury, endotoxemia and stress.

The research protocol includes detailed food intake analysis, including timing and composition, markers related to the neuroendocrine pathway cortisol, and observations of individually experienced symptoms. Moreover, the study also explores intestinal injury and endotoxemia parameters, such as I-FABP, CLDN-3, and LBP. As a first goal, this study investigates the timing and composition of food intake concerning changes in gut integrity, cortisol levels, and gastrointestinal symptoms. Investigators explored whether an athlete's nutritional intake influences any GI disturbance induced by high-intensity exercise. As a second goal, Investigators tried to connect symptoms and markers from both pathways with gastrointestinal complaints. Investigators hypothesised that cortisol levels would be more profound than other studied parameters in gastrointestinal symptoms.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Endothelial Dysfunction

Interventions

2000 meter ergometer testOTHER

Rowers performed 2000m ergometer test.A 2,000-m time trial is a standard test used to assess performance in rowers.

Eligibility

Sex: MALEMin age: 18 YearsMax age: 23 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Membership in Polish Rowing Team, * minimum five years of training, * total training time minimum of 240 minutes, * filling out a food diary, * finishing 2000-meter ergometer test. Exclusion Criteria: * Probiotics within the last three months, * prebiotics within the last three months * antibiotic therapy within the last three months, * dietary regime, * gastrointestinal diseases, * lactose intolerance

Locations (1)

Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow

Gorzów Wielkopolski, Lubusz Voivodeship, Poland

Outcomes

Primary Outcomes

I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury

I-FABP concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period

Claudin to measure tight junction leakage

Zonulin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

LBP (lipopolysaccharide binding protein) to measure endotoxin

LBP concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays

Lactic acid to measure fatigue after the race

Lactate concentration measured in capillary blood from earlobe \[mmol/L\] using a commercially available kit (Diaglobal, Berlin, Germany)

Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test

Corisol

Stress hormoneconcentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

Data from ClinicalTrials.gov

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