Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions from Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections that will be obtained under complete aseptic precautions.
Study Type
OBSERVATIONAL
Enrollment
100
Faculty of medicine Sohag university hospital
Sohag, Egypt
RECRUITINGIsolation and identification of pseudomonas aeruginosa causing different nosocomial infections.
Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions
Time frame: 6 months
Identification of antibiotic susceptibility pattern of isolated strains of pseudomonas
Antibiotic susceptibility testing of the isolates will be performed using disc diffusion method according to clinical laboratory standards institute (CLSI) guidelines
Time frame: 6 months
Detection of biofilm forming isolates
Detection of biofilm forming isolates by tissue culture plate method.
Time frame: 6 months
Detection of virulence and antibiotic resistance genes of pseudomonas aeruginosa.
Detection of virulence and antibiotic resistance genes by conventional Polymerase Chain Reaction
Time frame: 6 months
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.