Rationale: Collagen protein is the central structural component of extracellular connective tissues within skeletal muscle, bone, cartilage and skin. Dietary collagen peptides are a promising protein source to deliver the specific amino acid precursors required to support an increase in connective tissue protein synthesis across several tissues (e.g. muscle, skin). However, the digestion and absorption kinetics of multiple boluses of collagen peptides and the subsequent impact on muscle and skin connective tissue protein synthesis rates have not yet been assessed in vivo in humans. Objective: To assess the impact of ingestion of multiple boluses of collagen peptides on muscle connective and skin protein synthesis in vivo in humans. Study design: Double-blind, parallel-group, placebo-controlled intervention study. Study population: 20 healthy young males, aged 18-35 years. Intervention : Participants will perform unilateral resistance exercise followed by the ingestion of either 100 g of collagen peptides (in boluses) or a non-caloric placebo (flavoured water) drinks, while all drinks will contain vitamin C. Continuous intravenous stable isotope amino acid tracer infusions will be applied, plasma, skin and muscle samples will be collected in order to assess protein synthesis rates in skin and muscle tissue. Main study parameters/endpoints: Primary study parameters are muscle connective protein synthesis rates. Secondary study parameters are skin and myofibrillar protein synthesis rates, plasma amino acid concentrations and body composition.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
TRIPLE
Enrollment
27
Ingestion of 40, 20, 20 and 20 gram of collagen protein right after and every 2 hours after a bout of exercise
A single resistance exercise session of the leg press and leg extension exercise
Maastricht University Medical Centre
Maastricht, Netherlands
Muscle connective protein synthesis rates rested leg
The primary analysis will be an independent t-test, comparing postprandial muscle connective protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the rested condition between groups.
Time frame: one value calculated over 8 hours
Muscle connective protein synthesis rates exercised leg
Independent t-test, comparing postprandial muscle connective protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the exercised condition between groups.
Time frame: one value calculated over 8 hours
Myofibrillar protein synthesis rates rested leg
Independent t-test, comparing postprandial myofibrillar protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the rested condition between groups.
Time frame: one value calculated over 8 hours
Myofibrillar protein synthesis rates exercised leg
Independent t-test, comparing postprandial myofibrillar protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the exercised condition between groups.
Time frame: one value calculated over 8 hours
Skin protein synthesis rates
Independent t-test, comparing postprandial skin protein synthesis rates over the 0-8 h period (i.e., one integrated value).
Time frame: one value calculated over 8 hours
Plasma insulin concentrations
Plasma insulin concentrations
Time frame: measured over the 8 hour post-prandial period
Plasma amino acids concentrations
Plasma amino acids concentrations
Time frame: measured over the 8 hour post-prandial period
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