Senescent Endometrial Cells in Patients with Thin Endometrial Lining

Trio Fertility100 enrolled

Overview

The goal of this observational study is to determine if thin endometrial lining that is unresponsive to estrogen might be associated with the presence of senescent cells in patients following long-term use of oral contraceptives. The main question it aims to answer is: Are there any senescent cells present in thin endometrial lining? During the luteal phase of the cycle, participants will will undergo: * Gynecologic US to measure the endometrial lining thickness and pattern. * Endometrial biopsy with Pipelle catheter

The investigators hypothesized that senescent cells in the endometrium may be the cause of the failure of thin endometrium to respond to normal growth stimuli such as estrogen. The outcome that research team anticipate to measure is the percentage of senescent cells in the endometrial biopsies of women with thin endometrium compared to the control group biopsies.

Study Type

OBSERVATIONAL

Enrollment

100

Conditions

Asherman's Syndrome Thin Endometrial Lining Cell Senescence

Interventions

An endometrial biopsy is the removal of a small piece of tissue from the endometrium, which is the lining of the uterus. Endometrial biopsies are commonly done to check for the window of implantation, to determine ovulation or for enhancing implantation (procedure called endometrial scratching). For this purpose, we will use a "Pipelle" catheter. This procedure is very common in gynecologic practice and generally considered safe. In most of the cases this procedure is indicated for evaluation of irregular vaginal bleeding, vaginal bleeding in post-menopausal women and for infertility related problems (recurrent implantation failures for example).

Eligibility

Sex: FEMALEMin age: 18 YearsMax age: 39 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Age 18-39 * BMI - up to 35 * Thin endometrium after OCP use * Asherman's syndrome * Women already undergoing endometrial biopsy for infertility testing (eg. EMMA testing) * Women with archived endometrial biopsy samples Exclusion Criteria: * Women with systemic disease (Rheumatic disease, DM) * Women with coagulopathies * Women with active pelvic inflammatory process * Uterine anomalies * Pregnant women * Any hormonal contraception use or IUD

Outcomes

Primary Outcomes

Senescent cells presence

Percentage of senescent cells in the endometrial biopsies of women with thin endometrium compared to the control group biopsies.

Time frame: Through study completion, an average of 3 years.