Bronchial asthma affects more than a quarter of a billion people worldwide. It is responsible for over 1000 deaths a day, of which the majority are preventable (Levy, 2015). Asthma is a common heterogeneous disease characterized by chronic airway inflammation. It is defined by the history of variable respiratory symptoms, such as shortness of breath, chest tightness, wheezes, and cough, together with variable expiratory airflow limitation. Airflow limitation may later become persistent (GINA, 2023). The diagnosis of asthma is based on the history of these characteristic symptoms and evidence of variable expiratory airflow limitation from bronchodilator reversibility testing or other tests (GINA, 2023).
Study Type
OBSERVATIONAL
Enrollment
194
RNA extraction (isolation from whole blood) II-Synthesis of CDNA from RNA by reverse transcription III-Gene expression assay by RT-PCR reaction
Sohag University Hospital
Sohag, Egypt
Long non-coding MALAT1 gene expression assay:
Gene expression assay by RT-PCR reaction
Time frame: 2 MONTHS
TNF-alpha
measured in serum by ELISA
Time frame: 2 MONTHS
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