Clinical evaluation of vaccines against respiratory viruses is currently based on the analysis of systemic immune responses, whereas respiratory immunity is the first line of defense against respiratory pathogens. In addition to secretory immunoglobulin A (IgA) in mucosal fluids which are essential to neutralize the pathogens at mucosal surfaces, tissue-resident memory immune cells have been shown to be crucial in protection. Furthermore, memory immune cells in blood able to migrate to airway tissues also play a crucial role. Airway immune responses have not been studied a lot due to the lack of a standardized methodology to evaluate them in humans.
The goal of this study is to develop a methodology to collect and analyze nasal tissue-resident memory T and B cells and to evaluate peripheral memory T and B cells expressing airway homing markers in healthy volunteers. Three devices for nasal cell sampling will be compared. Tissue-resident and peripheral memory immune responses will be determined using flow cytometry and correlated with humoral and cellular responses, as well as with gene expression at mucosal and systemic levels. .
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
DIAGNOSTIC
Masking
SINGLE
Enrollment
30
Nasal tissue-resident memory T and B cells are quantified using flow cytometry.
Nasal fluid is collected using strip (Hunt Development) in both nostrils
Saliva is collected using Oracol (Malvern Medical Developments)
Isolation of peripheral blood mononuclear cells (PBMC) and serum from blood
ELISA to measure antibody responses in mucosal fluids and blood
Neutralization test to measure neutralizing antibody responses in mucosal fluids and blood to measure neutralizing antibody responses in mucosal fluids and blood
ELISPot to measure cellular responses in blood
Transcriptomics to quantify the gene expression of immunological markers in blood
Centre Hospitalier Universitaire
Saint-Etienne, France
Frequency of resident memory lymphocytes in the nasal mucosa measured by FLOQSwab
This outcome is measured by the three devices : * FLOQSwab * Rhino-Pro Curette * Cervical brush
Time frame: At inclusion, one month, two months
Frequencies of resident memory T and B lymphocytes
Frequencies of resident memory T and B lymphocytes are measured by the three devices : * FLOQSwab * Rhino-Pro Curette * Cervical brush
Time frame: At inclusion, one month, two months
Expression levels of respiratory mucosal migration markers on peripheral memory lymphocytes
This outcome is measured by the three devices : * FLOQSwab * Rhino-Pro Curette * Cervical brush
Time frame: At inclusion, one month, two months
Visual analog scale for pain
Scale from 0 to 10 (0 = no pain, 10 = intolerable pain) This outcome is measured by the three devices : * FLOQSwab * Rhino-Pro Curette * Cervical brush
Time frame: At inclusion, one month, two months
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