The purpose of this research is to explore what types of microbes are present on garden-fresh versus store-bought fruits and vegetables, as well as how they might affect the human gut microbiome.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
20
Participants are asked to consume the USDA-recommended amounts of fruits and vegetables harvested from their gardens.
Participants are asked to consume the USDA-recommended amounts of fruits and vegetables purchased from a supermarket.
Institute for Health in the Built Environment, University of Oregon
Eugene, Oregon, United States
RECRUITINGDifference in fecal bacterial abundance, as characterized by qPCR with "universal" bacterial primers
ANOVA statistical test of bacterial gene copies (a proxy for absolute abundance) used to compare whether Garden-Fresh Produce and Supermarket Produce interventions differentially affect fecal bacterial abundance.
Time frame: Baseline, 1 week, 3 weeks
Difference in fecal microbial diversity, as characterized by shotgun metagenomics.
ANOVA statistical test of Shannon index used to compare whether Garden-Fresh Produce and Supermarket Produce interventions differentially affect fecal microbial diversity.
Time frame: Baseline, 1 week, 3 weeks
Difference in fecal microbial composition, as characterized by shotgun metagenomics.
PERMANOVA statistical test used to compare whether Garden-Fresh Produce and Supermarket Produce interventions differentially affect fecal microbial composition.
Time frame: Baseline, 1 week, 3 weeks
Presence of differentially abundant microbial taxa, as characterized by shotgun metagenomics.
Negative binomial GLM statistical test used to identify microbial taxa associated with either Garden-Fresh Produce or Supermarket Produce intervention.
Time frame: Baseline, 1 week, 3 weeks
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