The goal of this clinical trial is to know if a novel sperm selection device (CA0) has a similar performance than the standard selection method in teratozoospermic and azoospermic men. The main questions it aims to answer are: Does the CA0 sperm selection device increases the sperm concentration? Does the CA0 sperm selection device increases the sperm motility? What are the differences in sperm membrane potential when CA0 is used? Do intracellular Calcium levels change when CA0 is used? Does CA0 increase fertilization rates? Researchers will compare CA0 to the standard of care for sperm selection (density gradient centrifugation) to see if CA0 has similar performance Participants will: Provide a semen sample Have their semen sample analyzed, processed and used for assisted reproduction technologies
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
SUPPORTIVE_CARE
Masking
SINGLE
Enrollment
115
Sperm separation with density gradients
Sperm separation with a novel device
CITMER
Mexico City, Mexico
Sperm membrane potential
Evaluation of the KCl-induced difference of membrane potential voltage in mV, after sperm selection.
Time frame: Within 2 hours from the sperm collection
Sperm intracellular Calcium
Evaluation of the progesterone-induced intracellular calcium fluorescent peaks after sperm selection
Time frame: Within 2 hours from the sperm collection
Sperm concentration
Assessment of sperm count after sperm separation
Time frame: Within 2 hours from the sperm collection
Sperm motility
Percentage of progresive, non-progressive and immotile sperm after sperm separation
Time frame: Within 2 hours from the sperm collection
Fertilization rates
Determination of the rate of 2 pronucleous, 2 polar bodies zygotes after insemination
Time frame: 17-20 hours after insemination by IVF or ICSI
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