This study investigates the effects of melatonin supplementation on cumulus cell gene expression and in vitro fertilization (IVF) outcomes in women over 35 years old. In the study group, patients will receive melatonin supplementation for at least two months prior to their IVF cycles. Cumulus cells will be collected after oocyte retrieval, and IVF outcomes will be assessed.
Aging in female reproductive cells, particularly cumulus granulosa cells, is associated with various dysfunctions that compromise fertility. Melatonin, a hormone primarily produced by the pineal gland, is renowned for its multifaceted roles in regulating physiological processes, including sleep-wake cycles, immune function, and antioxidative defense. Melatonin's antioxidant action is mediated through direct scavenging of free radicals, upregulation of antioxidant enzymes, and improvement of mitochondrial efficiency. These properties make melatonin a promising candidate for mitigating the adverse effects of aging on cellular function. This study aims to explore the effects of melatonin supplementation on cumulus cell gene expression and in vitro fertilization (IVF) outcomes in women over 35 years old. In the study group, patients will receive melatonin supplementation for at least two months prior to their IVF cycles. In the control group, patients will proceed directly to IVF cycles without melatonin supplementation. Cumulus cells will be collected after oocyte retrieval, and gene expression will be measured. Additionally, clinical pregnancy rate, live birth rate, and miscarriage rate between the two groups will be evaluated.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
TREATMENT
Masking
NONE
Enrollment
200
In the study group, patients will receive melatonin supplementation for at least two months prior to IVF cycles
Department of Obstetrics and Gynecology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan
Kaohsiung City, Taiwan
RECRUITINGLive birth rate (%)
a viable infant after 24 weeks of gestation
Time frame: through study completion, an average of 4 year
oxygen consumption rate of mitochodria (%)
to assess mitocondrial function in cumulus cells
Time frame: through study completion, an average of 4 year
mRNA levels of cumulus cell genes
to assess cumulus cells gene expression analysis
Time frame: through study completion, an average of 4 year
Clinical pregnancy rate (%)
the presence of a fetal heartbeat detected via transvaginal sonography at 6-7 weeks of gestation
Time frame: through study completion, an average of 4 year
Ongoing pregnancy rate (%)
the continuation of the pregnancy beyond 12 weeks of gestation
Time frame: through study completion, an average of 4 year
Amount of ATP production (moles/min) of mitochodria
to assess mitocondrial function in cumulus cells
Time frame: through study completion, an average of 4 year
Kuan-Hao Tsui
CONTACT
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