Fecal Microbiota and Monocyte Trained Immunity in CHD Patients With Different hsCRP

Union Hospital, Tongji Medical College, Huazhong University of Science and Technology100 enrolled

Overview

This single-center study enrolled patients aged 35-75 years who were diagnosed with unstable angina (UA), and had at least one of the three major coronary arteries with a ≥ 50% reduction in lumen diameter by angiographic visual estimation. Patients were all recruited at the Department of Cardiology, Union Hospital, Wuhan, Hubei Province, China. Exclusion criteria included acute myocardial infarction, prior myocardial infarction, malignancies, auto-immune or auto-inflammatory diseases, or ongoing infection. Feces and blood samples were collected on admission were used promptly for ex vivo studies. the aim of the study is to investigate whether the fecal microbiota and peripheral blood monocytes in patients with coronary heart disease at different levels of inflammation are associated with the level of inflammation.

Study Type

OBSERVATIONAL

Enrollment

100

Conditions

Coronary Heart Disease Unstable Angina

Interventions

Eligibility

Sex: ALLMin age: 35 YearsMax age: 75 Years

Medical Language ↔ Plain English

Inclusion Criteria: 1. Age \>35 and \<75 years 2. Admitted for UA and had at least one of the three major coronary arteries with ≥50% reduction in lumen diameter by angiographic visual estimation 3. Informed consent obtained Exclusion Criteria: 1. Prior myocardial infarction 2. Left ventricular ejection fraction ≤ 40% on echocardiography 3. Have a history of malignancies, auto-immune, auto-inflammatory disease or on immunomodulatory medication 4. Ongoing acute, chronic, or concurrent infectious disease 5. Surgery 3 months before sample collection 6. Immunization with a vaccine within 4 weeks before sample collection 7. Pregnancy, or breast feeding 8. History of primary or secondary immunodeficiency 9. Renal failure with estimated glomerular filtration rate (eGFR) \< 60 mL/min/1.73m2 10. Active liver disease with alanine aminotransferase (ALT \>80 U/L) or aspartate aminotransferase (AST \> 80 U/L) 11. History of chronic obstructive pulmonary disease (COPD) 12. Refuse to provide written consent 13. Any other circumstances in which the investigator judges that the patient is not suitable

Outcomes

Primary Outcomes

blood monocytes trained immunity

1.Blood monocytes were detected by flowcytometry to evaluate the monocyte subsets and CCR2 expression; 2. monocytes were enriched from blood cells and used for ex vivo lipopolysaccharide stimulation, mRNA sequencing and ATAC sequencing; 3.The metabolic bias of monocytes was detected by Seahorse technology and metabolics.

Time frame: within 3 days

Secondary Outcomes

fecal microbiome profile

the feccal microbiota was detected by 16s rRNA sequencing, and the analyses includes alpha and beta diversity, the differential microbiota between two groups, and the correlation between microbiota and clinical characteristics including age, BMI, blood lipids, and other available continous variables.