The main goal of this study is to find out if supplementing with resveratrol improves skeletal muscle adaptations to strengthening exercise (resistance training or strength training) in adults age 60+, and to determine whether circulating vehicles of inter-organ communication (extracellular vesicles) and their cargo might help explain those responses. The investigators will also examine whether resveratrol supplementation improves blood vessel function and cognitive function (brain health) in response to strength training.
After screening and providing informed consent, participants (healthy older adults, age 60+, minimally active) will be familiarized with the procedures and undergo pre-testing. After pre-testing, participants will be assigned to supplement with resveratrol or placebo (double-blinded) daily while undergoing a strength training intervention. Strength training will be performed 3x/week for two 6-week blocks, and each visit will be supervised by a member of the study team. An additional week of testing will follow each of the two 6-week blocks.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
TRIPLE
Enrollment
36
After pre-testing, participants will perform full-body strength training three times per week for two 6-week blocks. The acute program variables (sets, repetitions, training frequency) will follow a non-linear periodized, progressive design consisting of muti-joint and single-joint exercises. Exercise stress will be varied by using different exercises and loads. The two training blocks will be separated by a mid-intervention testing week, allowing for the study team to monitor participants' progress, communicate progress to participants to promote adherence, and adjust target loads as appropriate for the second half of the intervention. An additional testing week will follow the second 6-week block.
Participants will be randomized to consume resveratrol (500 mg) daily for 14 weeks (two 6-week blocks of resistance training \[supervised, full-body, 3 days/week\] + one testing week \[3 days\] following each block) after the pre-testing period. Participants will be provided with 7 days' worth of supplements at a time in pre-filled pill boxes. Pill boxes will be refilled weekly and adherence monitored.
Department of Kinesiology & Sport Management, Texas Tech University
Lubbock, Texas, United States
RECRUITINGCirculating extracellular vesicle microRNA profile
Circulating extracellular vesicles will be isolated from plasma and their microRNA cargo will be sequenced.
Time frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Muscle torque
Participants will be instructed to try and straighten their leg, against resistance, as forcefully as possible and maximal torque of the quadriceps assessed using a dynamometer.
Time frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Muscle fatigability
Participants will perform 32 repetitions of knee extensions on a dynamometer and the change in torque from the beginning to the end will be used to indicate fatigability.
Time frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Muscle strength
Participants will perform submaximal testing to assess strength on key exercises included in the strength training protocol.
Time frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Appendicular skeletal muscle mass index
Assessed using dual-energy x-ray absorptiometry (DEXA)
Time frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Waist-to-hip ratio
Waist and hip circumferences measured using measuring tape
Time frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Vascular function
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Participants will be randomized to consume placebo capsules (cellulose, visually identical to resveratrol capsules) daily for 14 weeks (two 6-week blocks of resistance training \[supervised, full-body, 3 days/week\] + one testing week \[3 days\] following each block) after the pre-testing period. Participants will be provided with 7 days' worth of supplements at a time in pre-filled pill boxes. Pill boxes will be refilled weekly and adherence monitored.
Flow-mediated dilation
Time frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Cognitive function
Repeatable Battery for the Assessment of Neuropsychological Status (RBANS)
Time frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Grip strength
Handgrip dynamometry
Time frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Lower body strength/endurance
Chair stand test (30 seconds)
Time frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Gait speed
4-m walk test
Time frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)