Regional Adipose Tissue Characteristics in Type II Diabetes

Concordia University, Montreal140 enrolled

Overview

Fat tissue in different parts of the body has different characteristics. Several studies have associated abdominal fat to an increased risk for multiple metabolic diseases, such as type 2 diabetes and cardiovascular disease, compared with fat located under the skin at the hips and thighs. However, the investigators do not know how the abdominal fat causes or contributes to the development of these diseases or how the development of these disease may in turn cause or contribute to changes in the fat tissue. The general objective of this study is to understand these mechanisms. To investigate these questions, the study will recruit 140 participants, both men and women aged 18-65 years. The participants will be divided into three groups: 40 people with obesity, 40 with diabetes, and 60 lean individuals. Participants will be recruited from patients at the CIUSSS du Nord-de-l'Île-de Montréal - Hôpital du Sacré-Coeur de Montréal who are awaiting bariatric or general abdominal surgery, as well as from the general population in the Montreal area.

Study Type

OBSERVATIONAL

Enrollment

140

Conditions

Obesity and Type 2 Diabetes Bariatric Patients Undergoing Bariatric Surgery Adipose Tissue Dysfunction Type 2 Diabetes Mellitus Bariatric Surgery Adipose Tissue Inflammation

Eligibility

Sex: ALLMin age: 18 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Men, premenopausal and postmenopausal females between 18-65 years * Anticipating having bariatric surgery (participants with obesity) or general surgery in the abdominal area (normal weight participants) with a duration of less than 1.5 hours. * BMI 30 kg / m2 (participants with obesity) * Weight Stable for a minimum of two months prior to the first pre-op visit Exclusion Criteria: * Smoking * Renal impairment defined by creatinine clearance; 60 ml/minute * Uncontrolled hypothyroidism * No comorbidity other than diabetes type II Exceptions: controlled hypertension, hypothyroidism controlled, controlled dyslipidemia * Use prescribed medication that affect our outcomes E.g. Exceptions include metformin, DPP-4 inhibitors, inhibitors of the α-glucosidase, hypotensive with a stable dose for 3 months lipid lowering with a stable dose for 3 months.

Outcomes

Primary Outcomes

Immune Cell Quantification

Flow Cytometry will be used to quantify immune cells in the fat tissue.

Time frame: 5 years

Pre-adipocytes and adipocyte characteristics

Cellular characteristics such as proliferation, differentiation and cellular senescence will measured.

Time frame: 5 years

Blood Inflammatory Markers

The inflammatory cytokines (e.g. IL6, TNFα), chemokines (e.g. monocyte chemoattractant protein-1), genomics, and adipokines (e.g. leptin, adiponectin) in blood and adipose tissue via PCR, luminex or ELISA will be measured

Time frame: 5 years

Regional Adipose Tissue Characteristics in Type II Diabetes

Overview

Conditions

Eligibility

Locations (1)

Outcomes

Primary Outcomes

Central Contacts