Physical Activity Level and Sleep Quality in Type 2 Diabetes

Selcuk University80 enrolled

Overview

In this study, we investigated the effects of physical activity level and sleep quality level on total antioxidant level (TAS), total oxidant level (TOS), Malondialdehyde (MDA) and Superoxide Dismutase (SOD) which are markers of oxidative stress, irisin, fibronectin type III-protein 5 (FNDC5).

Study Type

OBSERVATIONAL

Enrollment

Conditions

Type 2 Diabetes Mellitus (T2DM)Sleep Biomarkers / Blood Physical Activity Level

Interventions

Biomarkers / bloodOTHER

Analysis of routine biochemical parameters

QuestionnaireOTHER

Physical activity level will be assessed using the International Physical Activity Questionnaire Short Form (IPQI) and sleep quality will be assessed using the Pittsburgh Sleep Quality Index (PSQI). The data to be obtained from blood will be obtained by selecting blood samples that will be sent to biological waste.

Eligibility

Sex: ALLMin age: 30 YearsMax age: 65 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Being between 30-65 years old * Volunteering to participate in the study * Being literate * Being diagnosed with Type 2 Diabetes Exclusion Criteria: * Having a physical or mental illness that prevents communication * malignant disease, * infectious disease, * chronic obstructive pulmonary disease, * liver disease, * patients with kidney disease and joint or muscle disease will not be included

Locations (1)

Selcuk University

Konya, Selcuklu, Turkey (Türkiye)

Outcomes

Primary Outcomes

biomarkers derived from blood sera

blood glucose, total cholesterol, triglycerides, LDL and HDL analyzes

Time frame: 10 Minutes

ELISA method of oxidative stress markers

All reagents of the ELISA kits of the oxidative stress markers TAS, TOS, MDA and SOD will be brought to room temperature. Standards will be prepared according to the protocol and added to a 96-well plate with the samples. Buffer solution will be added to each well and incubated for 90 min at room temperature. After each step, necessary washes will be performed and finally chromogenic agents will be added and read spectrophotometrically. Although the timing and additions may vary according to the parameter, in general the procedure is as indicated. Each sample will be run 2 times for both TAS and TOS

Time frame: 10 minutes

Analysis of exercise-related markers by ELISA method

Time frame: 10 minutes

International Physical Activity Questionnaire

Time frame: 5 minutes

Pittsburgh Sleep Quality Index

Time frame: 5 minutes

Data from ClinicalTrials.gov

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