Immunity Markers in Intensive Care Patients and Ventilator-associated Pneumonia

Centre Hospitalier Intercommunal Aix-Pertuis40 enrolled

Overview

The goal of this observational study is to show the direct correlation between the occurrence of recurrence of VAP and postagressive immunoparalysis, monitored by HLA-DR rate below litterature-acknowledged threshold, in a well conducted antibiotherapy context, in patient admitted in the Intensive Care Unit. The main questions it aims to answer are: * evaluation of the association between death and persistence of immunoplegia using HLA-DR monitoring * search an association between immunoplegia depth and severity of the initial state of shock * search an association between immunoplegia depth and viral reactivation * compare association of immunoplegia duration and HLA-DR nadir and VAP occurrence Blood samples will be taken from participants to HLA-DR dosage, at the time of inclusion and once a week then.

The occurrence of ICU-acquired infections in patients admitted to the intensive care unit (ICU) results in increased morbidity and mortality, increased length of stay in the ICU, and also clearly increased healthcare costs. The incidence of these infections fluctuates between 15% and 40%, depending on the study. A major problem in the ICU is the recurrence and relapse of ventilator-associated pneumonia (VAP), with increased exposure to antibiotics and a probable increase in average length of stay. One of the possible hypothesis that could explain relapses/recurrences of VAP is incorrect conducted antibiotherapy. To prevent this, in the unit, we currently perform antibiotics pharmacological assays and adapt them to the antibiogram. Another possible explanation to treatment failure could be patients' postagressive immunoparalysis. It has clearly been demonstrated that postagressive immunoparalysis is a predisposing state to healthcare related infections. Some markers can be used to monitor this immunoplegia state. Several studies have shown that low HLA-DR expression and reduced CD16 expression (polymorphonuclear neutrophils percentage) is associated with increased susceptibility to develop infections in the ICU. Immunity monitoring could be an interesting tool to identify populations most at risk of developing healthcare-associated infections after a state of shock, and could become an interesting line of thinking for the use of immunomodulatory therapies. To best evaluate these therapies and find a place for them in the current arsenal, it is essential to integrate them into daily practice by linking them to a significant clinical event, such as recurrent healthcare-associated infections, despite properly conducted antibiotic treatment.

Study Type

OBSERVATIONAL

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Patient over 18 years old * Patient admitted in the Intensive Care Unit of the CHIAP * Patient under mechanical ventilation * Patient with infectious pneumonia * Informed Consent Form (ICF) obtained from the patient or emergency ICF obtained from close relatives * Patient beneficiary of French social security, whatever the regime Exclusion Criteria: * Patient under 18 years old * Patient with severe neutropenia (neutrophils \< 0.5 G/L) * Patient under immunosuppressive treatment * Use of corticosteroids (intravenous or oral) prior to ICU admission * Use of therapeutic antibodies * Onco-hematological disease (e.g. lymphoma, leukemia...) under treatment or treated in the 5 years prior to inclusion * End of chemotherapy 6 months prior to inclusion * Patients with innate or acquired immune deficiency (e.g., severe combined immunodeficiency, HIV or AIDS, at any stage) * Patients with a decision to limit or discontinue active therapies, at the time of inclusion * Patients with an estimated ICU stay of less than 48 hours * Participation in an interventional study * Patient deprived of their liberty * Patient under tutorship or curatorship * Pregnant or breastfeeding woman

Outcomes

Primary Outcomes

Occurrence of a VAP recurrence/relapse

Number of occurrence of a VAP recurrence/relapse

Time frame: From date of hospital admission until the date of the end of hospitalization or date of death from any cause, whichever came first, assessed up to 100 months

Secondary Outcomes

Viral reactivation

To search for association between immunoplegia depth and viral reactivation (CMV)

Time frame: From date of hospital admission until the date of the end of hospitalization or date of death from any cause, whichever came first, assessed up to 100 months

Association between immunoparalysis depth and state of shock severity within the first 24 hours

To search for association between immunoplegia depth and severity of the initial state of shock (lactatemia, amine dosage, SOFA score over 48 hours)

Time frame: First 24 hours

Persistence of immunoparalysis during hospitalization and care-related infections

To compare association of immunoplegia duration and HLA-DR nadir and VAP occurrence

Time frame: From date of hospital admission until the date of the end of hospitalization or date of death from any cause, whichever came first, assessed up to 100 months

HLA-DR nadir and link with care-related infections

To compare association of immunoplegia duration and HLA-DR nadir and VAP occurrence

Time frame: From date of hospital admission until the date of the end of hospitalization or date of death from any cause, whichever came first, assessed up to 100 months

Immunity Markers in Intensive Care Patients and Ventilator-associated Pneumonia

Overview

Conditions

Interventions

Eligibility

Locations (1)

Outcomes

Primary Outcomes

Secondary Outcomes

Central Contacts