Primary Objective: use immunofluorescence (IF) on previously obtained biopsies to analyze and quantify changes in adipose tissue of subjects injected with poly-L-lactic acid (PLLA) or saline.
Sixteen female adults that participated in SPLASH study will be enrolled in this retrospective study. It aims to analyze previously collected biopsy samples from participants who completed the "SPLASH: Split-Body Randomized Clinical Trial of Poly-L-Lactic Acid for Adipogenesis and Volumization of the Hip Dell" study. The study will use immunohistology to assess changes in adipose tissue associated with treatment with PLLA (poly-L-lactic acid). We hypothesize that specimens treated with PLLA will be found to have more adipocyte-derived stem cells and preadipocytes undergoing mitosis and differentiation compared to control specimens.
Study Type
OBSERVATIONAL
Enrollment
14
Analyze previous biopsies to quantify changes induced by Poly-L-Lactic Acid in Adipose Tissue
Cosmetic Laser Dermatology/West Dermatology Research Center
San Diego, California, United States
Change in cell count per area between PLLA and saline-treated specimens.
Analyzing between PLLA and saline-treated specimens for the following populations: * adipocyte derived stem cells * proliferating adipocyte-derived stem cells * preadipocytes/adipocyte precursors * proliferating preadipocytes/adipocyte precursors * mature adipocytes and actively differentiating precursors The following proteins will be quantified and the differences between PLLA and saline-treated specimens at 6 months compared to pre-treatment will also be analyzed: * β-catenin * PPARγ * Adiponectin * Perilipin-1
Time frame: Specimens are from 6 months post-treatment compared to pre-treatment / Baseline
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