This study investigates the physiological responses and the metabolic burden of a Pilates Reformer strength training session in physically inactive premenopausal women with a body mass index of 18-24.9 and 25.01-39.9. The sample included 20 women divided into two groups: 10 with normal weight (NW) and 10 with overweight/obesity (OB). In a non-randomised control design, the participants will perform a 60-minute muscle-strengthening session with Pilates Reformer equipment.
Pilates Reformer (PR) is one of the most popular types of exercise worldwide and is considered a gentle and safe method for untrained and overweight people. The PR training approach has been found to contribute positively to various health conditions (vascular function, blood pressure, physical fitness, musculoskeletal problems), improving the quality of life and mental well-being of the participants. At the same time, it seems that it can have a positive effect on the lipid profile and other biochemical markers such as insulin and fasting glucose. Furthermore, muscle strengthening training with PR equipment provides better levels of mobility, increasing active range of motion, and also provides greater pelvic and trunk stability for both adult and middle-aged participants. This study is designed to investigate the physiological and metabolic burden of a muscle-strengthening session with PR equipment in 20 pre-menopausal and physically inactive women with a body mass index (BMI) of 18-24, 99 \& 25.01-39.9, as well as to determine the contribution of energy systems (mitochondrial, glycolytic) during and after the exercise. Each training session will last approximately 60 minutes, will be led by at least one trainer, and will include a warm-up of 7-10 minutes, muscle strengthening and mobility exercises of 35 minutes, and a recovery of 5 minutes. Anthropometric indicators, dietary intake, resting heart rate, blood lactate, resting metabolic rate, muscle soreness, rate of perceived exertion, blood pressure, and performance measurements will be measured at baseline. The metabolic cost during PR training session will be estimated from heart rate, blood lactate, resting oxygen uptake, exercise oxygen uptake, and excess post-exercise oxygen consumption measurements using a portable gas analyzer. After the PR training session will be measured the rate of perceived exertion, blood pressure, blood lactate, and muscle soreness levels.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
OTHER
Masking
SINGLE
Enrollment
20
Participants will perform a 60-minute Pilates Reformer muscle-strengthening session. The training will include a 7-10 minute warm-up, 35 minutes of muscle strengthening and mobility exercises and a 5-minute recovery. During the training, the physiological responses and the energy expenditure of the participants will be recorded.
Department of Physical Education and Sport Science
Trikala, Thessaly, Greece
Department of Physical Education and Sports Science, University of Thessaly
Trikala, Thessaly, Greece
Change in exercise-induced energy expenditure
Exercise energy expenditure (kcal) will be measured using a portable indirect calorimetry system
Time frame: At pre-exercise, 60 minutes during PR training, and 30 minutes after the exercise session (single bout lasting 60 minutes)
Change in excess post-exercise oxygen consumption (EPOC)
EPOC (kcal) will be measured using a portable indirect calorimetry system
Time frame: At 30 minutes after the exercise session (single bout lasting 60 minutes)
Change in blood lactate concentration (BLa)
BLa (mmol/L) concentration will be measured in a microphotometer with commercially available kits
Time frame: At pre- and post-exercise session (single bout) at 4 minutes post-exercise
Change in heart rate
Heart rate (bpm) will be measured with a wearable heart rate monitor
Time frame: At pre-exercise, 60 minutes during PR training, and 30 minutes after the exercise session (single bout lasting 60 minutes)
Change in perceived exertion
Rating of perceived exertion (RPE) will be measured with the Borg scale (0-10)
Time frame: At pre- and post-exercise session (single bout) at 4 minutes post-exercise.
Respiratory Exchange Ratio (RER)
Respiratory Exchange Ratio (RER) will be measured using a portable indirect calorimetry system
Time frame: At pre-exercise, 60 minutes during PR training, and 30 minutes after the exercise session (single bout lasting 60 minutes)
Breath Frequency (BF)
Breath Frequency (BF) will be measured using a portable indirect calorimetry system
Time frame: At pre-exercise, 60 minutes during PR training, and 30 minutes after the exercise session (single bout lasting 60 minutes)
Change in oxygen consumption
Oxygen consumption will be measured using a portable indirect calorimetry system
Time frame: At pre-exercise, 60 minutes during PR training, and 30 minutes after the exercise session (single bout lasting 60 minutes)
Body weight
Body weight will be measured on a beam balance with a stadiometer
Time frame: At baseline and at 40 minutes after the exercise session.
Body height
Body height will be measured on a beam balance with a stadiometer
Time frame: At baseline
Body mass index (BMI)
BMI will be calculated using the Quetelet's equation
Time frame: At baseline
Waist circumference (WC)
WC (cm) will be measured using a Gullick II tape
Time frame: At baseline
Hip circumference (HC)
HC (cm) will be measured using a Gullick II tape
Time frame: At baseline
Waist-to-hip ratio (WHR)
WHR will be calculated by dividing the waist by the hip measurement
Time frame: At baseline
Resting metabolic rate (RMR)
RMR (kcal) will be measured using a portable open-circuit indirect calorimeter with a ventilated hood system
Time frame: At baseline
Body fat (BF)
Body fat (%) will be assessed by whole-body dual-energy X-ray absorptiometry (DXA)
Time frame: At baseline
Fat mass (FM)
FM (kg) will be assessed by whole-body dual-energy X-ray absorptiometry (DXA)
Time frame: At baseline
Fat-free mass (FFM)
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FFM (kg) will be assessed by whole-body dual-energy X-ray absorptiometry (DXA)
Time frame: At baseline
Bone mass density
Bone mass density will be measured by using Dual-emission X-ray absorptiometry
Time frame: At baseline
Bone mass content
Bone mass content will be measured by using Dual-emission X-ray absorptiometry
Time frame: At baseline
Peak Maximal oxygen consumption (Peak VO2)
Peak VO2 will be estimated by open circuit spirometry via breath by breath method
Time frame: At baseline
Dietary intake
Dietary intake will be assesed over a 7-day period using diet recalls
Time frame: At baseline
Systolic Blood Pressure
Systolic Blood Pressure will be examined using a sphygmomanometer
Time frame: At pre-exercise, 60 minutes during PR training, and 30 minutes after the exercise session (single bout lasting 60 minutes)
Diastolic Blood Pressure
Diastolic Blood Pressure will be examined using a sphygmomanometer
Time frame: At pre-exercise, 60 minutes during PR training, and 30 minutes after the exercise session (single bout lasting 60 minutes)
Rest Heart Rate
The rest heart rate will be estimated using a heart rate monitor
Time frame: At baseline
Evaluation of physical activity (PA)
The PA will be evaluated for seven days before the intervention via accelerometry
Time frame: At baseline