Beetroot juice is a rich source of nitrate (NO3-), which serves as a precursor to nitric oxide (NO) via the NO3- to nitrite (NO2-) to NO conversion pathway. This pathway is believed to enhance vasodilation and improve neuromuscular function, such as by increasing sarcoplasmic reticulum calcium release and re-uptake, leading to enhanced force production in type II muscle fibers and improved muscle contractile recruitment. Therefore, the aim of this study was to investigate the effects of beetroot juice supplementation on muscle contractile properties, as assessed by tensiomyography, and neuromuscular performance in sprint-trained athletes.
Sports nutrition is a rapidly expanding scientific field that has experienced a significant increase in interest from the academic community over the past decade. Despite the extensive marketing of numerous nutritional products that claim to optimize athlete health, function, and performance, only a limited number of sports foods or dietary supplements have been supported by robust evidence for enhancing performance. Among these, caffeine, creatine, beetroot juice, β-alanine, and bicarbonate are notable for their validated efficacy, though the effectiveness of these supplements can vary based on the specific event, context of use, and the individual athlete's goals and responsiveness. Beetroot juice, in particular, is a rich source of nitrate (NO3-), which serves as a precursor to nitric oxide (NO) through the NO3- to nitrite (NO2-) to NO conversion pathway. This pathway is believed to promote vasodilation and improve neuromuscular function, such as by increasing sarcoplasmic reticulum calcium release and re-uptake, leading to enhanced force output in type II muscle fibers and improved muscle contractile recruitment. Therefore, the aim of this study was to investigate the effects of beetroot juice supplementation on muscle contractile properties, as assessed by tensiomyography, and neuromuscular performance in sprint-trained athletes. The goal was to gain a deeper understanding of the physiological effects induced by beetroot juice intake in short-distance track and field disciplines.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
QUADRUPLE
Enrollment
12
Player randomization was performed using an online tool (https://www.randomizer.org/). An independent researcher assigned alphanumeric codes to each sequence to ensure blinding of both sprint athletes and researchers during the trials. Each player participated in two identical experimental trials separated by one week to allow physical recovery between testing occasions and washout from the beetroot juice treatment.
Universidad Pontificia Comillas
Madrid, Spain
Tensiomyography changes (seconds): Delay time (Td) ; Contraction time (Tc) ; Sustain time (Ts) ; Relaxation time (Tr)
The measurement of the rectus femoris, biceps femoris, gastrocnemius lateralis, and gastrocnemius medialis muscles was conducted using tensiomyography. We analyzed different parameters such as selay time (Td) as a time between the electrical impulse and 10% of the contraction; Contraction time (Tc) as a time between 10% and 90% of the contraction; Sustain time (Ts) as a time between 50% of the contraction and 50% of the relaxation; Relaxation time (Tr) as a time between 90% and 50% of the relaxation.
Time frame: 1-week
Changes in 60-m and 100-m sprint test (seconds)
Time to complete 60-m and 100-m sprint test using photocell timing gates
Time frame: 1-week
Changes in maximal countermovement and squat jump height (cm)
Maximal jump height using a contact platform
Time frame: 1-week.
Changes in nitrites and nitrates levels in saliva (μmol/L)
Using specific kit ELISA analysis
Time frame: 1-week
Changes in side effects questionnarie
Questionnarie based on side-effects related to beetroot juice ingestion (A scale consisting of nine items, each answered with "yes" or "no")
Time frame: 1-week
Changes in rate of perception effort
Using rate of perceived exertion scale (1-10 points)
Time frame: 1-week
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