Effects of Intrauterine Flushing With Human Chorionic Gonadotropin on ICSI Outcome

Omayma Ismail Khalaf90 enrolled

Overview

a number of infertile women who were prepared for ICSI and immediately post ova pick up intrauterine flushing with human chorionic gonadotropin was done to study the effect of this drug on ICSI outcome and ultrasound parameter.

Ninety infertile women included. Written informed consent was obtained before being included in the study. Three groups of patients according to type of intervention. Assessment of plasma hormone level was done for all. The quantification of serum TNF and IL-10 levels was conducted through the application of the enzyme-linked immunosorbent assay (ELISA) methodology at a privately-owned laboratory.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

TREATMENT

Masking

DOUBLE

Enrollment

Conditions

Female Infertility

Interventions

hCGDRUG

observe hCG effect on endometrial receptivity

Eligibility

Sex: FEMALEMin age: 18 YearsMax age: 40 Years

Medical Language ↔ Plain English

Inclusion Criteria: 1. The patient\'s consent to participate in the research study is crucial for data collection and analysis. 2. Each pair of individuals undergoing IVF/ICSI procedures typically possess grade 1 (G1) embryos during the embryo transfer process. 3. The age range of the female participants varied from 18 to 40 years. 4. Infertility arising from female factors, including issues like blocked fallopian tubes, lack of ovulation, and unexplained fertility challenges, was examined. 5. Male-related factors contributing to infertility were also taken into consideration. 6. The procedure involved the transfer of grade one embryos during a fresh cycle. Exclusion Criteria: Endometrioses, whether clinically suspected or discovered during laparoscopy, are of interest. 2\. Instances of congenital abnormalities in the reproductive system are being examined. 3\. Individuals above the age of forty are under consideration. 4. Individuals who do not possess G1 embryos are also included. 5. Patients with chronic systemic conditions (such as diabetes, hematologic disorders, hypertension, autoimmune disorders of the connective tissue, asthma, thyroid disorders) are a part of the study. 6\. The presence of empty follicles is noted in stimulated cycles where no oocytes were obtained during the aspiration procedure. \-

Locations (1)

High Institute for Infertility Diagnosis and Assisted Reproductive Technology

Baghdad, Iraq

Outcomes

Primary Outcomes

Serum Level of TNF-alfa,IL-10, for All Participants in Three Study Group at Day of Ova Pickup Compared to That at Day of Embryo Transfer

All participants in the study had a blood sample taken on the day of ovum retrieval (within 10 minute after ova pickup) for the purpose of measuring the level of interleukin-10 and TNF-alfa in the blood, and a second sample was taken on the day of embryo transfer (ten minute before embryo transfer) to asses both marker,

Time frame: at day of ova pickup a blood sample aspirated, and on day of embryo transfer about 3-5 days post ova pickup blood sample taken within ten minute before embryo transfer to asses serum level of TNF-alfa and IL-10 for all participent

Ultrasound Parameter (RI,PI)

assessment of sub-endometrial blood flow indicis immediately within 10 minute post ova pickup and second exam within ten minute before embryo transfer day which is within 3-5 days of ova pickup there are no maximum or minimum value for the parameter to be measured and lower value is better these measure was performed by using ultrasound device

Time frame: Within 10 minutes after ova pickup, ten minute before embryo transfer using ultrasound device

Systole Flow/ Diastole Flow

ultrasound parameter to asses endometrial receptivity by measuring sub-endometrial flow systole / diastole ratio

Time frame: within ten minute post ova pickup and ten minute prior to embryo transfer which is done within 3-5 days post ova pickup

TNF-alfa/IL-10 Ratio

ratio of serum level of TNA-alfa /IL-10 at time of ova pickup and at day of embryo transfer

Time frame: within ten minute post ova pickup and ten minute prior to embryo transfer which is done within 3-5 days post ova pickup

Secondary Outcomes

Intra Cytoplasmic Sperm Injection Outcome

Number of patients who get pregnant after ICSI in each study group

Data from ClinicalTrials.gov

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