Obesity is a risk factor for chronic diseases, and improving energy expenditure is a way to prevent and treat obesity. The purpose of this project is to explore the relationship between the effect of coffee on energy expenditure and caffeine metabolism, which has important significance in the rational application of caffeine.
The investigators will study the relationship between the effect of coffee on energy expenditure and caffeine metabolism. The investigators will recruit healthy participants. Participants will be forbidden to consume stimulant drinks such as coffee for 24 hours before the experiment. When the participants arrive at the lab on the day of the experiment, they will sign an informed consent form, a health history questionnaire and a daily coffee consumption questionnaire. The changes of drinking water/coffee on total energy expenditure (TEE), body temperature, heart rate, blood pressure, electrocardiogram, memory，cognition, sleep time and so on will be observed. The concentration of caffeine and its metabolites in the blood and urine will be measured by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS).
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
60
Participants will drink the same volume of water or coffee, caffeine content is 2mg/kg by body weight.
Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
Shenzhen, Guangdong, China
RECRUITINGTotal energy expenditure
Total energy expenditure will be measured by Chamber (OMNICAL).
Time frame: 2 days
The concentration of caffeine and its metabolites in urine
The contents of caffeine and it's metabolites will be measured by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS ).
Time frame: 2 days
The concentration of caffeine and its metabolites in serum
The concentration of caffeine and its metabolites in serum will be measured by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS ).
Time frame: 1 day
Body temperature
Body temperature of participants will be measured by body temperature patch (RIT-P02-MED).
Time frame: 2 days
Electrocardiogram (ECG)
ECG of participants will be measured by Amu ECG belt, the changes in P wave, PR interval, PR segment, QRS wave group, J point, ST segment, T wave, QT interval, and U wave will be observe.
Time frame: 2 days
Blood pressure
Systolic and diastolic blood pressure of participants will be measured by Omron digital sphygmomanometer.
Time frame: 2 days
Height
Height will be measured by seca 217 stable stadiometer. Subjects wearing no shoes.
Time frame: The morning of the first day of the experiment, 5 mins
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Body weight
Fasting body weight will be measured by Bioimpedance Analysis (TANITA, MC-980).
Time frame: The morning of the first day of the experiment, 5 mins
Fat mass
Fat mass will be measured by Magnetic Resonance Imaging (Shanghai united imaging, uMR 790 ).
Time frame: The morning of the first day of the experiment, 10 mins
Fat free mass
Fat free mass will be Bioimpedance Analysis (Tanita, MC-980).
Time frame: on the morning of the experiment, 5 mins
Heart rate
Heart rate will be measured by Polar heart rate belts.
Time frame: 2 days
Hunger rating
Hunger score will be measured on (Visual Analogue Scale) VAS by a pen and a paper. According to the scores of each participant, the highest score was 100 % hunger rate, and hunger rate (%) at other time points will be obtained from hunger score × 100 % / the highest score.
Time frame: 2 days
Memory and cognitive tests
Memory and cognitive tests will be tested by Cognitive Experiment Standard Toolbox (CEST).
Time frame: The morning of the experiment, 60 mins
Physical activity
The physical activity of volunteers will be recorded by accelerometer (GT3XP-BTLE).
Time frame: 7 days
Sleep duration
The sleep duration of volunteers will be recorded by polar watch.
Time frame: 2 days
Waist circumferences
Waist circumferences will be measured using a whole body laser scanner (TG2000-F).
Time frame: The morning of the first day of the experiment,10 mins.