Cellular and Molecular Analysis of Synovial Tissue of Patients With Arthritis

N/ANot Yet RecruitingNCT06737952

University Hospital, Basel, Switzerland90 enrolled

Overview

This study aims to study the inflammatory response in various forms of arthritis.

Arthritis, a significant heald burden is characterized by joint inflammation that severely impacts patients' quality of live. Despite advancements in treatment, many individuals with conditions like rheumatoid arthritis (RA), osteoarthritis (OA), and spondyloarthritis (SpA) do not achieve satisfactory outcomes, underscoring the need for more precise therapeutic strategies. The project aims to characterize the inflammatory response in various form of arthritis by collecting and analyzing synovial tissue-the connective tissue lining joints. Biopsies will be collected through ultrasound-guided biopsies (UGSB), a minimally invasive and well-tolerated procedure. Presence of immune and stromal cells in the tissue, their interactions, and their relationship to clinical symptoms are characterized by histological and molecular methods. By comparing these features across different forms of arthritis, the study aims to identify molecular pathways and biomarkers that distinguish these diseases and their subtypes. The study hopes to contribute to a broader understanding of arthritis pathophysiology, ultimately translating into novel therapeutic targets and tailored clinical strategies.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Arthritis Arthritis, Rheumatoid Osteoarthritis Spondyloarthritis Synovitis

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Age \>18 years * Clinically relevant arthritis in one or more joints as assessed by the treating rheumatologist, including RA, SpA, connective tissue disease and OA. Exclusion Criteria: * Known allergies to local anesthetics * Family history of bleeding, history of increased bleeding tendency or prolonged bleeding, current treatment with oral anticoagulants or platelet aggregation inhibitors; Quick \< 65%, international normalised ratio (INR) ≥ 1.3, thrombocyte count \< 100'000/μl, activated partial thromboplastin time (aPTT) \> 37 sec * Inability or insufficient knowledge of project language to understand the information given in the informed consent form

Outcomes

Primary Outcomes

Grade of synovitis

assessed by the Krenn score in the hematoxylin and eosin staining (Score 0-3, 0=normal, 3=severe)

Time frame: immediately after collection to study completion, up to 5 years

classification pathotypes

immunohistochemical staining with cell type specific markers including CD15, CD68, CD3, CD20, CD138 of the collected tissues from different arthritis subtypes

Time frame: immediately after collection to study completion, up to 5 years

comparing transcriptomic signature

single-cell RNA sequencing of collected tissues from different arthritis subtypes to identify disease specific biomarkers.

Time frame: immediately after collection to study completion, up to 5 years

Central Contacts

Diego Kyburz, Prof. Dr.

CONTACT

+41 61 328 74 81 diego.kyburz@usb.ch

Data from ClinicalTrials.gov

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