The INSPIRE CHILD-study is a clinical research project focused on understanding lung diseases in children. The study aims to explore how the presence of certain bacteria, immune responses, and inflammatory markers in the lungs are connected to different lung diseases in pediatric patients. Background: Lung diseases in children can have long-lasting effects on their health, not only during childhood but also as they grow into adults. However, the specific causes and mechanisms behind these diseases are not yet fully understood. The INSPIRE CHILD-study is designed to investigate whether children with various lung conditions have distinct differences in the types of bacteria, immune system activity, and inflammation in their lungs. Hypothesis: Different lung diseases in children are hypothesized to be associated with unique patterns of bacteria, immune responses, and inflammation in the lungs By studying these patterns, the investigators hope to gain new insights that could lead to better diagnostic tools and more personalized treatments for children suffering from lung diseases. Children already scheduled to undergo bronchoscopy are included in the study. A small sample of fluid from the lungs is collected, and the sample is analyzed for bacteria, immune markers, and signs of inflammation. This research could pave the way for improved care and treatment for young patients with lung conditions.
Study Type
OBSERVATIONAL
Enrollment
200
No intervention
Childrens Center for Pulmonary and Allergic diseases, Aarhus University Hospital
Aarhus N, Denmark
Cytokines
Legendplex assay for measuring cytokines in bronchoalveolar lavage fluid and plasma (e.g. IL-1-beta, IL-1RA, IL-2, IL-6, IL-8, IL-10, IL-17, IL-18, IL-33, IL-35, TGF-beta, TNF-alpha)
Time frame: Day 0 (Subsequent to study inclusion)
Immunophenotype
Using flow cytometry to investigate immune cells: e.g. Neutrophils, Eosinophils, Monocytes, NK-cells, B-cells, CD4+-T-cells, CD8+-T-cells
Time frame: Day 0 (Subsequent to study inclusion)
Lung microbiome
We seek to investigate whether it is feasible to map the microbiome, virome and mycobiome of the pediatric lung from BAL-fluid. The study will investigate and report findings of bioinformatics analysis.
Time frame: Day 0 (Subsequent to study inclusion)
Concentration range for selected interleukins in BAL-fluid
Time frame: Day 0 (Subsequent to study inclusion)
Lung disease association
Can we associate a specific profile of findings with a particular pediatric lung disease?
Time frame: Through study completion, an average of 2 year
Clinical symptoms
2 weeks prior to date of inclusion/bronchoscopy, the subjects medical history is reviewed for the following: Characterization of cough Respiratory distress Functional limitations (due to respiratory distress) (acc. to age) Fever (temperature above 38.0 C) Breath sounds Urgent need for medication
Time frame: 2 weeks prior to enrollment
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