The aim of this study is to evaluate the acute effects of beetroot juice and caffeine, consumed individually or in combination, on lower-limb strength and muscular endurance in physically active women. The main questions it aims to answer are: * Does the co-ingestion of beetroot juice and caffeine enhance lower-limb strength and muscular endurance more than either substance alone or a placebo? * How do the acute effects of beetroot juice interact with caffeine on physical performance? The study was conducted using a randomized, crossover design to evaluate the following four conditions: Beetroot juice with placebo Caffeine with placebo Beetroot juice with caffeine Placebo only Each participant completed all conditions, with a sufficient washout period between sessions to eliminate potential carryover effects. This design allowed for a direct comparison of the acute effects of each supplementation strategy on lower-limb strength and muscular endurance. Participant Protocol Participants consumed their assigned supplement on a single testing day for each condition. After supplementation, they performed standardized tests to measure lower-limb strength and muscular endurance. Progress was monitored, and data from all conditions were collected to assess the acute effects of each intervention.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
DOUBLE
Enrollment
15
* 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session * 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session
\- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session
\- 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session
\- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session
Edificio IMIBIC Avda Menéndez Pidal s/n
Córdoba, Spain
Change of height reached in jump
Evaluated through Countermovement jump test with a recovery period of 45 seconds between jumps. The height reached was recorded in centimeters.
Time frame: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
Movement velocity
The velocity was recorded in meters per second (m/s) during two repetitions of the back squat, evaluated at 50% and 75% of the repetition maximum (RM).
Time frame: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
Muscular endurance test
The test consists of three exercises: back squat, leg press and leg extension. Three series will be performed until concentric failure at 75% RM, three minutes of rest between each series (3 series). The number of repetitions was recorded.
Time frame: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
Rating of perceived exertion (RPE).
Immediately at the end of the muscular endurance test, participants will be instructed to report their RPE using a scale 1-10 (worse to better outcome).
Time frame: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
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