Purpose: To optimize the preparation process of probiotic powder and assess its impact on healthy athletes, providing a scientific basis for functional foods. Procedures: Probiotic Powder Preparation: Using vacuum freeze-drying to improve bacterial survival rates. Human Trials: Recruiting healthy athletes aged 20-30, divided into placebo and probiotic powder groups for an 8-week trial. Testing and Analysis: Conducting running exhaustion tests and blood biochemical analysis to evaluate the effects of probiotic powder. Importance: Understanding the effects of probiotics on athletes helps develop better health products.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
SINGLE
Enrollment
60
Arm Description: Randomly select 30 healthy and well-trained athletes aged between 20 and 30 to serve as the control group. Participants in the control group will undergo an 8-week placebo supplementation intervention. The placebo has a similar appearance and taste to the probiotic powder but does not contain active probiotic ingredients. Before the intervention begins, a comprehensive health assessment and exercise capacity test will be conducted for the participants. Blood samples will be collected for baseline biochemical marker analysis. Participants will be instructed to take the placebo once a day at the same time to ensure the consistency and standardization of the intervention. Participants will be asked to record their daily consumption, including the time of intake, any omissions, or discomfort. After the 8-week intervention, exercise capacity tests and blood sample collections will be conducted again. Comparing exercise performance and biochemical markers before and after t
Experimental: Arm Description: Randomly select 30 healthy and well-trained athletes aged between 20 and 30 to serve as the experimental group. Participants in the experimental group will undergo an 8-week probiotic powder supplementation intervention. The dosage of the probiotic powder is set at 1x10\^9 CFU/kg·bw·d, which means ingesting 10 billion CFU (Colony-Forming Units) of probiotics per kilogram of body weight per day. Before the intervention begins, a comprehensive health assessment and exercise capacity test will be conducted for the participants. Blood samples will be collected for baseline biochemical marker analysis. Participants will be instructed to take the probiotic powder once a day at the same time to ensure the consistency of the dosage and the standardization of the intervention. Participants will be asked to record their daily consumption, including the time of intake, any omissions, or discomfort. After the 8-week intervention, exercise ca
Lactic acid
Lactic acid：Collecting 2 ml of venous blood for measurement indicators
Time frame: 8 weeks
Creatine kinase
Creatine kinase: Collecting 2 ml of venous blood for measurement indicators.
Time frame: 8 weeks
Blood urea nitrogen
Blood urea nitrogen: Collecting 2 ml of venous blood for measurement indicators.
Time frame: 8 weeks
Liver glycogen
Liver glycogen: Collecting 2 ml of venous blood for measurement indicators.
Time frame: 8 weeks
Muscle glycogen
Muscle glycogen: Collecting 2 ml of venous blood for measurement indicators.
Time frame: 8 weeks
Total superoxide dismutase
Total superoxide dismutase: Collecting 2 ml of venous blood for measurement indicators.
Time frame: 8 weeks
Glutathione
Glutathione: Collecting 2 ml of venous blood for measurement indicators.
Time frame: 8 weeks
Malondialdehyde
Malondialdehyde
Time frame: 8 weeks
Maximum Oxygen Uptake
The cardiopulmonary function testing system provided by the German CORTEX company measures the maximum oxygen uptake.
Time frame: 8 weeks
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