Molar-Incisor Hypomineralization Vs Periodontal Health

Recep Tayyip Erdogan University Training and Research Hospital80 enrolled

Overview

Background: To investigate the effect of molar incisor hypomineralization (MIH) on periodontal health, to evaluate the relationship between gingival crevicular fluid (GCF) volume, and GCF biomarkers such as matrix metalloproteinase-8 (MMP-8), matrix metalloproteinase-20 (MMP-20), and transforming growth factor-beta 1 (TGF-β1), and MIH. Methods: The study was conducted on 40 pediatric patients diagnosed with MIH (Study Group/SG), and 40 pediatric patients healthy for MIH (Control Group/CG). Clinical periodontal measurements (plaque index (PI), gingival index (GI), probing pocket depth (PPD), and GCF volume were performed. Concentrations of MMP-8, MMP-20, and TGF-β1 in GCF were determined using enzyme-linked immunosorbent assay (ELISA). Mann-Whitney U test and T-test were used for comparisons between groups. Significance was set at p=0.05 for statistical analysis.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Molar-Incisor Hypomineralization Peridontal Disease

Interventions

Clinical evaluation

In this study, it was aimed to measure the levels of MMP-8, MMP-20, and TGF-β1 in GCF together with the volume of GCF to investigate the effect of MIH on oral hygiene, and gingival health, and also to evaluate the relationship between MIH, and various biomarkers.

Eligibility

Sex: ALLMin age: 8 YearsMax age: 14 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * the ages of 8 and 14 who were diagnosed with MIH in their maxillary permanent first molars, * had healthy maxillary permanent first molars, * scored 3 (positive) or 4 (definitely positive) on the Frankl Behavior Scale, * had no mental or physical disability, * did not need urgent treatment and/or were not in pain, * agreed to participate in the study with their parents and signed the consent form. Exclusion Criteria: * Children with systemic, chronic, and/ or metabolic diseases, * under active orthodontic treatment, with developmental defects of enamel (excluding MIH), or born prematurely

Outcomes

Primary Outcomes

Periodontal indexes measurement

PI, GI, and PPD measurements were performed to evaluate the periodontal health of the children participating in the study.

Time frame: 1 day

GCF measurement

In the study, GCF was collected in the morning hours (between 09:00 and 11:00) one day after the patient's clinical periodontal measurements were complete.

Time frame: 1 day

Evaluation of MMP-8, MMP-20 and TGF-β1 Levels by ELISA Method

The levels of MMP-8 (Elabscience® EL-H1450, Biotechnology Co., Ltd, Wuhan, China), MMP-20 (Bioassay Technology Laboratory, Cat. No E4139Hu), and TGF-β1 (Elabscience® EL-H0110, Biotechnology Co., Ltd, Wuhan, China) in GCF samples were measured using ELISA kits.

Time frame: for three days from the beginning of the study