Pregnancy increases the risk of thrombosis. Placenta-mediated diseases are a risk factor for cardiovascular pathologies and can lead to maternal-fetal morbidity and mortality. It is essential to understand the cellular and molecular mechanisms of dysfunctions at the vascular-placental interface so that systemic vascular risk can be characterized and, ultimately, screened for, on the basis of new markers (targeted preventive management). Deregulated autophagy could be the starting point for cell death by apoptosis or necrosis leading to complications. The pathophysiological mechanisms involved in trophoblast apoptosis are incompletely described. This project follows on from the GrossAuTop-1 study, which investigated the intra- and inter-individual variability of autophagy and apoptosis activities in women during pregnancy. The aim of this project is to study autophagy and apoptosis activities specifically in women developing a placental vascular complication during pregnancy.
Pregnancy increases the risk of thrombosis. Diseases mediated by the placenta are a risk factor for cardiovascular pathologies. They are responsible for significant maternal-fetal morbidity and mortality. Understanding and exploring the cellular and molecular mechanisms of dysfunctions at the vascular-placental interface could provide arguments for understanding systemic vascular risk, characterizing it and ultimately screening for it on the basis of new markers, thus paving the way for targeted preventive management, feeding into the general principle of precision medicine. Autophagy enables cell development, differentiation and survival, but if deregulated, it could be the starting point for cell death by apoptosis or necrosis, and promote the development of complications. The pathophysiological mechanisms involved in trophoblast apoptosis are incompletely described. A deregulation of the trophoblast proliferation/cell death balance could be at the origin of placental pathologies. The regulation of autophagy and autophagy-dependent events during pregnancy have not been fully identified. We hypothesize that there is an intratrophoblastic dialogue between autophagy and apoptosis mechanisms, with the promotion of one partially inhibiting the other. The increase in trophoblastic autophagy during pregnancy could thus constitute an anti-apoptosis defense phenomenon, whose depletion would lead to cellular apoptosis and pathogenic consequences when it devastates the syncytiotrophoblast. This project follows on from the GrossAuTop-1 study, which investigated the intra- and inter-individual variability of autophagy and apoptosis activities in women during pregnancy: the inclusions corresponded to all-pregnant women, the majority of whom developed a normal pregnancy. The aim of this project is to study autophagy and apoptosis activities specifically in women developing a placental vascular complication during pregnancy.
Study Type
OBSERVATIONAL
Enrollment
50
16 blood samples (16 tubes, i.e. 55.3 ml) will be taken at inclusion. Pregnant women will be seen every month as part of their pregnancy follow-up, and blood (11 tubes, i.e. 35.5 ml) and urine samples will be taken at each follow-up visit. At delivery, a systematic blood sample will be taken as part of the usual care, and an additional 11 tubes of blood (35.5 ml) will be taken.
Urine samples will be taken at the inclusion visit and at each follow-up visit.
Nimes University Hospital
Nîmes, France
RECRUITINGPercentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Level of autophagy in trophoblastic test cells quantified by the percentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Time frame: Baseline
Percentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Level of autophagy in trophoblastic test cells quantified by the percentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Time frame: Month 1
Percentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Level of autophagy in trophoblastic test cells quantified by the percentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Time frame: Month 2
Percentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Level of autophagy in trophoblastic test cells quantified by the percentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Time frame: Month 3
Percentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Level of autophagy in trophoblastic test cells quantified by the percentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Time frame: Month 4
Percentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Level of autophagy in trophoblastic test cells quantified by the percentage of cells expressing LC3 (microtubule-associated protein light chain 3) protein
Time frame: At delivery
Apoptosis activity
Percentage of cells expressing annexin-V in pregnant women developing placental vascular pathology
Time frame: Baseline
A. Apoptosis activity
Percentage of cells expressing annexin-V in pregnant women developing placental vascular pathology
Time frame: Month 1
A. Apoptosis activity
Percentage of cells expressing annexin-V in pregnant women developing placental vascular pathology
Time frame: Month 2
A. Apoptosis activity
Percentage of cells expressing annexin-V in pregnant women developing placental vascular pathology
Time frame: Month 3
A. Apoptosis activity
Percentage of cells expressing annexin-V in pregnant women developing placental vascular pathology
Time frame: Month 4
A. Apoptosis activity
Percentage of cells expressing annexin-V in pregnant women developing placental vascular pathology
Time frame: At delivery
B. Autophagy/apoptosis balance
Ratio between percentage of cells expressing LC3 protein and percentage of cells expressing annexin-V
Time frame: Baseline
B. Autophagy/apoptosis balance
Ratio between percentage of cells expressing LC3 protein and percentage of cells expressing annexin-V
Time frame: Month 1
B. Autophagy/apoptosis balance
Ratio between percentage of cells expressing LC3 protein and percentage of cells expressing annexin-V
Time frame: Month 2
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
B. Autophagy/apoptosis balance
Ratio between percentage of cells expressing LC3 protein and percentage of cells expressing annexin-V
Time frame: Month 3
B. Autophagy/apoptosis balance
Ratio between percentage of cells expressing LC3 protein and percentage of cells expressing annexin-V
Time frame: Month 4
B. Autophagy/apoptosis balance
Ratio between percentage of cells expressing LC3 protein and percentage of cells expressing annexin-V
Time frame: At delivery
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: LC3 protein
LC3 protein will be measured as a %
Time frame: Baseline
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: LC3 protein
LC3 protein will be measured as a %
Time frame: Month 1
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: LC3 protein
LC3 protein will be measured as a %
Time frame: Month 2
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: LC3 protein
LC3 protein will be measured as a %
Time frame: Month 3
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: LC3 protein
LC3 protein will be measured as a %
Time frame: Month 4
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: LC3 protein
LC3 protein will be measured as a %
Time frame: At delivery
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: cells expressing annexin-V
Cells expressing annexin-V will be measured as a %
Time frame: Baseline
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: cells expressing annexin-V
Cells expressing annexin-V will be measured as a %
Time frame: Month 1
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: cells expressing annexin-V
Cells expressing annexin-V will be measured as a %
Time frame: Month 2
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: cells expressing annexin-V
Cells expressing annexin-V will be measured as a %
Time frame: Month 3
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: cells expressing annexin-V
Cells expressing annexin-V will be measured as a %
Time frame: Month 4
C.Kinetics of different biological activities (autophagy, apoptosis, autophagy/apoptosis balance) during pregnancy follow-up: cells expressing annexin-V
Cells expressing annexin-V will be measured as a %
Time frame: At delivery
D. Concentration of circulating Placental Growth Factor
Circulating Placental Growth Factor measured on blood samples in pg/mL
Time frame: Baseline
D. Concentration of circulating Placental Growth Factor
Circulating Placental Growth Factor measured on blood samples in pg/mL
Time frame: Month 1
D. Concentration of circulating Placental Growth Factor
Circulating Placental Growth Factor measured on blood samples in pg/mL
Time frame: Month 2
D. Concentration of circulating Placental Growth Factor
Circulating Placental Growth Factor measured on blood samples in pg/mL
Time frame: Month 3
D. Concentration of circulating Placental Growth Factor
Circulating Placental Growth Factor measured on blood samples in pg/mL
Time frame: Month 4
D. Concentration of circulating Placental Growth Factor
Circulating Placental Growth Factor measured on blood samples in pg/mL
Time frame: At delivery
D. Concentration of circulating soluble fms-like tyrosine kinase receptor-1
Circulating soluble fms-like tyrosine kinase receptor-1 measured on blood samples in pg/mL
Time frame: Baseline
D. Concentration of circulating soluble fms-like tyrosine kinase receptor-1
Circulating soluble fms-like tyrosine kinase receptor-1 measured on blood samples in pg/mL
Time frame: Month 1
D. Concentration of circulating soluble fms-like tyrosine kinase receptor-1
Circulating soluble fms-like tyrosine kinase receptor-1 measured on blood samples in pg/mL
Time frame: Month 2
D. Concentration of circulating soluble fms-like tyrosine kinase receptor-1
Circulating soluble fms-like tyrosine kinase receptor-1 measured on blood samples in pg/mL
Time frame: Month 3
D. Concentration of circulating soluble fms-like tyrosine kinase receptor-1
Circulating soluble fms-like tyrosine kinase receptor-1 measured on blood samples in pg/mL
Time frame: Month 4
D. Concentration of circulating soluble fms-like tyrosine kinase receptor-1
Circulating soluble fms-like tyrosine kinase receptor-1 measured on blood samples in pg/mL
Time frame: At delivery
E. Correlation between autophagy/apoptosis balance and markers of renal function: proteinuria
Proteinuria will be measured in mg/L
Time frame: Baseline
E. Correlation between autophagy/apoptosis balance and markers of renal function: proteinuria
Proteinuria will be measured in mg/L
Time frame: Month 1
E. Correlation between autophagy/apoptosis balance and markers of renal function: proteinuria
Proteinuria will be measured in mg/L
Time frame: Month 2
E. Correlation between autophagy/apoptosis balance and markers of renal function: proteinuria
Proteinuria will be measured in mg/L
Time frame: Month 3
E. Correlation between autophagy/apoptosis balance and markers of renal function: proteinuria
Proteinuria will be measured in mg/L
Time frame: Month 4
E. Correlation between autophagy/apoptosis balance and markers of renal function: proteinuria
Proteinuria will be measured in mg/L
Time frame: At delivery
E. Correlation between autophagy/apoptosis balance and markers of renal function: creatinuria
Creatinuria will be measured in mmol/L
Time frame: Baseline
E. Correlation between autophagy/apoptosis balance and markers of renal function: creatinuria
Creatinuria will be measured in mmol/L
Time frame: Month 1
E. Correlation between autophagy/apoptosis balance and markers of renal function: creatinuria
Creatinuria will be measured in mmol/L
Time frame: Month 2
E. Correlation between autophagy/apoptosis balance and markers of renal function: creatinuria
Creatinuria will be measured in mmol/L
Time frame: Month 3
E. Correlation between autophagy/apoptosis balance and markers of renal function: creatinuria
Creatinuria will be measured in mmol/L
Time frame: Month 4
E. Correlation between autophagy/apoptosis balance and markers of renal function: creatinuria
Creatinuria will be measured in mmol/L
Time frame: At delivery
E. Correlation between autophagy/apoptosis balance and markers of renal function: C-reactive protein
C-reactive protein will be measured in mg/L
Time frame: Baseline
E. Correlation between autophagy/apoptosis balance and markers of renal function: C-reactive protein
C-reactive protein will be measured in mg/L
Time frame: Month 1
E. Correlation between autophagy/apoptosis balance and markers of renal function: C-reactive protein
C-reactive protein will be measured in mg/L
Time frame: Month 2
E. Correlation between autophagy/apoptosis balance and markers of renal function: C-reactive protein
C-reactive protein will be measured in mg/L
Time frame: Month 3
E. Correlation between autophagy/apoptosis balance and markers of renal function: C-reactive protein
C-reactive protein will be measured in mg/L
Time frame: Month 4
E. Correlation between autophagy/apoptosis balance and markers of renal function: C-reactive protein
C-reactive protein will be measured in mg/L
Time frame: At delivery
E. Correlation between autophagy/apoptosis balance and markers of renal function: Fibrinogen
Fibrinogen will be measured in g/L
Time frame: Baseline
E. Correlation between autophagy/apoptosis balance and markers of renal function: Fibrinogen
Fibrinogen will be measured in g/L
Time frame: Month 1
E. Correlation between autophagy/apoptosis balance and markers of renal function: Fibrinogen
Fibrinogen will be measured in g/L
Time frame: Month 2
E. Correlation between autophagy/apoptosis balance and markers of renal function: Fibrinogen
Fibrinogen will be measured in g/L
Time frame: Month 3
E. Correlation between autophagy/apoptosis balance and markers of renal function: Fibrinogen
Fibrinogen will be measured in g/L
Time frame: Month 4
E. Correlation between autophagy/apoptosis balance and markers of renal function: Fibrinogen
Fibrinogen will be measured in g/L
Time frame: At delivery
E. Coagulation parameters : activated partial thromboplastin time
Activated partial thromboplastin time will be measured in seconds
Time frame: Baseline
E. Coagulation parameters : activated partial thromboplastin time
Activated partial thromboplastin time will be measured in seconds
Time frame: Month 1
E. Coagulation parameters : activated partial thromboplastin time
Activated partial thromboplastin time will be measured in seconds
Time frame: Month 2
E. Coagulation parameters : activated partial thromboplastin time
Activated partial thromboplastin time will be measured in seconds
Time frame: Month 3
E. Coagulation parameters : activated partial thromboplastin time
Activated partial thromboplastin time will be measured in seconds
Time frame: Month 4
E. Coagulation parameters : activated partial thromboplastin time
Activated partial thromboplastin time will be measured in seconds
Time frame: At delivery
E. Coagulation parameters : Prothrombin time
Prothrombin time will be measured in seconds
Time frame: Baseline
E. Coagulation parameters : Prothrombin time
Prothrombin time will be measured in seconds
Time frame: Month 1
E. Coagulation parameters : Prothrombin time
Prothrombin time will be measured in seconds
Time frame: Month 2
E. Coagulation parameters : Prothrombin time
Prothrombin time will be measured in seconds
Time frame: Month 3
E. Coagulation parameters : Prothrombin time
Prothrombin time will be measured in seconds
Time frame: Month 4
E. Coagulation parameters : Prothrombin time
Prothrombin time will be measured in seconds
Time frame: At delivery
E. Coagulation parameters : Fibrinogen
Fibrinogen will be measured in g/L
Time frame: Baseline
E. Coagulation parameters : Fibrinogen
Fibrinogen will be measured in g/L
Time frame: Month 1
E. Coagulation parameters : Fibrinogen
Fibrinogen will be measured in g/L
Time frame: Month 2
E. Coagulation parameters : Fibrinogen
Fibrinogen will be measured in g/L
Time frame: Month 3
E. Coagulation parameters : Fibrinogen
Fibrinogen will be measured in g/L
Time frame: Month 4
E. Coagulation parameters : Fibrinogen
Fibrinogen will be measured in g/L
Time frame: At delivery
E. Coagulation parameters : D-dimers
D-dimers will be measured in ng/mL
Time frame: Baseline
E. Coagulation parameters : D-dimers
D-dimers will be measured in ng/mL
Time frame: Month 1
E. Coagulation parameters : D-dimers
D-dimers will be measured in ng/mL
Time frame: Month 2
E. Coagulation parameters : D-dimers
D-dimers will be measured in ng/mL
Time frame: Month 3
E. Coagulation parameters : D-dimers
D-dimers will be measured in ng/mL
Time frame: Month 4
E. Coagulation parameters : D-dimers
D-dimers will be measured in ng/mL
Time frame: At delivery
E. Coagulation parameters : Fibrin monomers
Fibrin monomers will be measured in mg/mL
Time frame: Baseline
E. Coagulation parameters : Fibrin monomers
Fibrin monomers will be measured in mg/mL
Time frame: Month 1
E. Coagulation parameters : Fibrin monomers
Fibrin monomers will be measured in mg/mL
Time frame: Month 2
E. Coagulation parameters : Fibrin monomers
Fibrin monomers will be measured in mg/mL
Time frame: Month 3
E. Coagulation parameters : Fibrin monomers
Fibrin monomers will be measured in mg/mL
Time frame: Month 4
E. Coagulation parameters : Fibrin monomers
Fibrin monomers will be measured in mg/mL
Time frame: At delivery
E. Coagulation parameters : Thrombin generation time
Thrombin generation time will be measured in seconds
Time frame: Baseline
E. Coagulation parameters : Thrombin generation time
Thrombin generation time will be measured in seconds
Time frame: Month 1
E. Coagulation parameters : Thrombin generation time
Thrombin generation time will be measured in seconds
Time frame: Month 2
E. Coagulation parameters : Thrombin generation time
Thrombin generation time will be measured in seconds
Time frame: Month 3
E. Coagulation parameters : Thrombin generation time
Thrombin generation time will be measured in seconds
Time frame: Month 4
E. Coagulation parameters : Thrombin generation time
Thrombin generation time will be measured in seconds
Time frame: At delivery
Complete Blood Count : White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils
White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils will be measured in 10\^9/L
Time frame: Baseline
Complete Blood Count : White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils
White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils will be measured in 10\^9/L
Time frame: Month 1
Complete Blood Count : White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils
White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils will be measured in 10\^9/L
Time frame: Month 2
Complete Blood Count : White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils
White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils will be measured in 10\^9/L
Time frame: Month 3
Complete Blood Count : White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils
White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils will be measured in 10\^9/L
Time frame: Month 4
Complete Blood Count : White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils
White blood cells, Lymphocytes, Neutrophils, Monocytes, Eosinophils and Basophils will be measured in 10\^9/L
Time frame: At delivery
Complete Blood Count : Red blood cells
Red blood cells will be measured in 10\^12/L
Time frame: Baseline
Complete Blood Count : Red blood cells
Red blood cells will be measured in 10\^12/L
Time frame: Month 1
Complete Blood Count : Red blood cells
Red blood cells will be measured in 10\^12/L
Time frame: Month 2
Complete Blood Count : Red blood cells
Red blood cells will be measured in 10\^12/L
Time frame: Month 3
Complete Blood Count : Red blood cells
Red blood cells will be measured in 10\^12/L
Time frame: Month 4
Complete Blood Count : Red blood cells
Red blood cells will be measured in 10\^12/L
Time frame: At delivery
Complete Blood Count : Hemoglobin
Hemoglobin will be measured in g/L
Time frame: Baseline
Complete Blood Count : Hemoglobin
Hemoglobin will be measured in g/L
Time frame: Month 1
Complete Blood Count : Hemoglobin
Hemoglobin will be measured in g/L
Time frame: Month 2
Complete Blood Count : Hemoglobin
Hemoglobin will be measured in g/L
Time frame: Month 3
Complete Blood Count : Hemoglobin
Hemoglobin will be measured in g/L
Time frame: Month 4
Complete Blood Count : Hemoglobin
Hemoglobin will be measured in g/L
Time frame: At delivery
Complete Blood Count : Platelets
Platelets will be measured in %
Time frame: Baseline
Complete Blood Count : Platelets
Platelets will be measured in %
Time frame: Month 1
Complete Blood Count : Platelets
Platelets will be measured in %
Time frame: Month 2
Complete Blood Count : Platelets
Platelets will be measured in %
Time frame: Month 3
Complete Blood Count : Platelets
Platelets will be measured in %
Time frame: Month 4
Complete Blood Count : Platelets
Platelets will be measured in %
Time frame: At delivery
Complete Blood Count : Hematocrit
Hematocrit will be measured in %
Time frame: Baseline
Complete Blood Count : Hematocrit
Hematocrit will be measured in %
Time frame: Month 1
Complete Blood Count : Hematocrit
Hematocrit will be measured in %
Time frame: Month 2
Complete Blood Count : Hematocrit
Hematocrit will be measured in %
Time frame: Month 3
Complete Blood Count : Hematocrit
Hematocrit will be measured in %
Time frame: Month 4
Complete Blood Count : Hematocrit
Hematocrit will be measured in %
Time frame: At delivery
Complete Blood Count : Mean Corpuscular Volume
Mean Corpuscular Volume will be measured in f/L
Time frame: Baseline
Complete Blood Count : Mean Corpuscular Volume
Mean Corpuscular Volume will be measured in f/L
Time frame: Month 1
Complete Blood Count : Mean Corpuscular Volume
Mean Corpuscular Volume will be measured in f/L
Time frame: Month 2
Complete Blood Count : Mean Corpuscular Volume
Mean Corpuscular Volume will be measured in f/L
Time frame: Month 3
Complete Blood Count : Mean Corpuscular Volume
Mean Corpuscular Volume will be measured in f/L
Time frame: Month 4
Complete Blood Count : Mean Corpuscular Volume
Mean Corpuscular Volume will be measured in f/L
Time frame: At delivery
Complete Blood Count : Mean Corpuscular Hemoglobin
Mean Corpuscular Hemoglobin will be measured in pg
Time frame: Baseline
Complete Blood Count : Mean Corpuscular Hemoglobin
Mean Corpuscular Hemoglobin will be measured in pg
Time frame: Month 1
Complete Blood Count : Mean Corpuscular Hemoglobin
Mean Corpuscular Hemoglobin will be measured in pg
Time frame: Month 2
Complete Blood Count : Mean Corpuscular Hemoglobin
Mean Corpuscular Hemoglobin will be measured in pg
Time frame: Month 3
Complete Blood Count : Mean Corpuscular Hemoglobin
Mean Corpuscular Hemoglobin will be measured in pg
Time frame: Month 4
Complete Blood Count : Mean Corpuscular Hemoglobin Concentration
Mean Corpuscular Hemoglobin Concentration will be measured in g/L
Time frame: Baseline
Complete Blood Count : Mean Corpuscular Hemoglobin Concentration
Mean Corpuscular Hemoglobin Concentration will be measured in g/L
Time frame: Month 1
Complete Blood Count : Mean Corpuscular Hemoglobin Concentration
Mean Corpuscular Hemoglobin Concentration will be measured in g/L
Time frame: Month 2
Complete Blood Count : Mean Corpuscular Hemoglobin Concentration
Mean Corpuscular Hemoglobin Concentration will be measured in g/L
Time frame: Month 3
Complete Blood Count : Mean Corpuscular Hemoglobin Concentration
Mean Corpuscular Hemoglobin Concentration will be measured in g/L
Time frame: At delivery
Ferritin
Ferritin will be measured in ng/mL
Time frame: Baseline
Ferritin
Ferritin will be measured in ng/mL
Time frame: Month 1
Ferritin
Ferritin will be measured in ng/mL
Time frame: Month 2
Ferritin
Ferritin will be measured in ng/mL
Time frame: Month 3
Ferritin
Ferritin will be measured in ng/mL
Time frame: Month 4
Ferritin
Ferritin will be measured in ng/mL
Time frame: At delivery
F. Association between autophagy/apoptosis balance and the presence of constitutive thrombophilia
Constitutive thrombophilia: presence of prothrombin or factor V Leiden gene mutation, protein S, C, antithrombin deficiency will be sought: YES/NO
Time frame: Baseline
F. Association between autophagy/apoptosis balance and the presence of constitutive thrombophilia
Constitutive thrombophilia: presence of prothrombin or factor V Leiden gene mutation, protein S, C, antithrombin deficiency will be sought: YES/NO
Time frame: Month 1
F. Association between autophagy/apoptosis balance and the presence of constitutive thrombophilia
Constitutive thrombophilia: presence of prothrombin or factor V Leiden gene mutation, protein S, C, antithrombin deficiency will be sought: YES/NO
Time frame: Month 2
F. Association between autophagy/apoptosis balance and the presence of constitutive thrombophilia
Constitutive thrombophilia: presence of prothrombin or factor V Leiden gene mutation, protein S, C, antithrombin deficiency will be sought: YES/NO
Time frame: Month 3
F. Association between autophagy/apoptosis balance and the presence of constitutive thrombophilia
Constitutive thrombophilia: presence of prothrombin or factor V Leiden gene mutation, protein S, C, antithrombin deficiency will be sought: YES/NO
Time frame: Month 4
F. Association between autophagy/apoptosis balance and the presence of constitutive thrombophilia
Constitutive thrombophilia: presence of prothrombin or factor V Leiden gene mutation, protein S, C, antithrombin deficiency will be sought: YES/NO
Time frame: At delivery
F. Association between autophagy/apoptosis balance and the presence of acquired thrombophilia
Acquired thrombophilia: presence of anti-phospholipid antibodies (lupus anticoagulant and/or anti-b2-glycoprotein 1 and/or anti-cardiolipid) will be sought: YES/NO
Time frame: Baseline
F. Association between autophagy/apoptosis balance and the presence of acquired thrombophilia
Acquired thrombophilia: presence of anti-phospholipid antibodies (lupus anticoagulant and/or anti-b2-glycoprotein1 and/or anti-cardiolipid) will be sought: YES/NO
Time frame: Month 1
F. Association between autophagy/apoptosis balance and the presence of acquired thrombophilia
Acquired thrombophilia: presence of anti-phospholipid antibodies (lupus anticoagulant and/or anti-b2-glycoprotein1 and/or anti-cardiolipid) will be sought: YES/NO
Time frame: Month 2
F. Association between autophagy/apoptosis balance and the presence of acquired thrombophilia
Acquired thrombophilia: presence of anti-phospholipid antibodies (lupus anticoagulant and/or anti-b2-glycoprotein 1 and/or anti-cardiolipid) will be sought: YES/NO
Time frame: Month 3
F. Association between autophagy/apoptosis balance and the presence of acquired thrombophilia
Acquired thrombophilia: presence of anti-phospholipid antibodies (lupus anticoagulant and/or anti-b2-glycoprotein 1 and/or anti-cardiolipid) will be sought: YES/NO
Time frame: Month 4
F. Association between autophagy/apoptosis balance and the presence of acquired thrombophilia
Acquired thrombophilia: presence of anti-phospholipid antibodies (lupus anticoagulant and/or anti-b2-glycoprotein 1 and/or anti-cardiolipid) will be sought: YES/NO
Time frame: At delivery
Comparison of results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance
The results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance) will be compared with data obtained from the first GrossAuTop-1 study (normal pregnancy).
Time frame: Baseline
Comparison of results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance
The results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance) will be compared with data obtained from the first GrossAuTop-1 study (normal pregnancy).
Time frame: Month 1
Comparison of results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance
The results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance) will be compared with data obtained from the first GrossAuTop-1 study (normal pregnancy).
Time frame: Month 2
Comparison of results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance
The results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance) will be compared with data obtained from the first GrossAuTop-1 study (normal pregnancy).
Time frame: Month 3
Comparison of results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance
The results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance) will be compared with data obtained from the first GrossAuTop-1 study (normal pregnancy).
Time frame: Month 4
Comparison of results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance
The results on trophoblastic cell autophagy induction potential, apoptosis and autophagy/apoptosis balance) will be compared with data obtained from the first GrossAuTop-1 study (normal pregnancy).
Time frame: At delivery
H.Association between autophagy/apoptosis balance and HbF levels in maternal blood during pregnancy.
Determination of fetal hemoglobin (HbF) in maternal blood, measured in %
Time frame: Baseline
H.Association between autophagy/apoptosis balance and HbF levels in maternal blood during pregnancy.
Determination of fetal hemoglobin (HbF) in maternal blood, measured in %
Time frame: Month 1
H.Association between autophagy/apoptosis balance and HbF levels in maternal blood during pregnancy.
Determination of fetal hemoglobin (HbF) in maternal blood, measured in %
Time frame: Month 2
H.Association between autophagy/apoptosis balance and HbF levels in maternal blood during pregnancy.
Determination of fetal hemoglobin (HbF) in maternal blood, measured in %
Time frame: Month 3
H.Association between autophagy/apoptosis balance and HbF levels in maternal blood during pregnancy.
Determination of fetal hemoglobin (HbF) in maternal blood, measured in %
Time frame: Month 4
H.Association between autophagy/apoptosis balance and HbF levels in maternal blood during pregnancy.
Determination of fetal hemoglobin (HbF) in maternal blood, measured in %
Time frame: At delivery