Blood DdPCR for Early Identification and Dynamic Surveillance of Pathogenic Bacteria in ICU Septic Patients: a Single-centre, Prospective, Observational Study

Union Hospital, Tongji Medical College, Huazhong University of Science and Technology60 enrolled

Overview

The purpose of this study is to investigate the efficacy of droplet digital PCR (ddPCR) in identifying pathogenic organisms in ICU sepsis patients, aiming to find a method that allows early identification of pathogenic organisms and dynamic surveillance in order to assess the association between pathogenic species and loads and clinical characteristics and outcomes.

This is a monocentric observational study. Patients admitted to the ICU with a first diagnosis of sepsis will be dynamically monitored for nucleic acid loads of pathogenic bacteria in the blood using ddPCR at different time points until the last blood sample is taken on day 28-30 or the patient is discharged from the ICU.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Sepsis Bloodstream Infections Critically Ill Intensive Care Unit Patients

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Patients with first diagnosis of sepsis after ICU admission Exclusion Criteria: * Age under 18 years * Do not meet Sepsis 3.0 diagnostic criteria * Refusal to sign informed consent

Locations (1)

Outcomes

Primary Outcomes

Nucleic acid load of pathogenic bacteria at different time points

Time frame: ''Nucleic acid load of pathogenic bacteria in the blood versus time'' could have a time frame of ''0, 2-3, 4-5, 6-7, 8-9, 10-12, 13-15, 20-22, 28-30 days post admission to the ICU with the first diagnosis of sepsis''.

Secondary Outcomes

28-day mortality

Time frame: Survival status at 28 days after the first diagnosis of sepsis on admission to the ICU