Samples from male partners of 120 infertile couples undergoing IVF were analyzed. Sperm morphology was assessed using the SpermBlue staining method, while TZI, MAI, and SDI were calculated. Embryo development was monitored until day 5 or 6. Statistical analyses included regression models and correlation coefficients to evaluate the predictive value of sperm indices on blastocyst development.
For spermatozoa selection we used ZyMot multi in all patients undergoing IVF. After intracytoplasmatic injection of spermatozoa we asses the blastocyst grading in day 5. NEQSI score meaning numerical embryonic quality score index was used for statistical purpose. The spermatozoa morphology indexes were: TZI (teratozoospermia index), MAI (multiple anomalies index), SDI (sperm deformity index) and analyzed with CASA analyzer. All these indexes were assessed in patients and correlated in all groups: patients that had a positive pregnancy test, patients that had a negative pregnancy rate, patients that had no blastocyst in day 5 and patients that had all aneuploid embyos.
Study Type
OBSERVATIONAL
Enrollment
120
We analyzed all sperm morphology indexes in all groups.
Calla Ivf Center
Oradea, Bihor County, Romania
Evaluation of sperm morphology indices on fertilization and blastulation rate
Functional sperm indices and embryo outcomes were evaluated to determine their relationship.
Time frame: 18 months
Evaluation of sperm morphology indices on IVF outcomes
The study's findings are limited to IVF populations and may not generalize to natural conception.
Time frame: 16 months
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