This cross-sectional study seeks to characterize the overall polyphenol metabotype in patients with T2DM in comparison to age-matched individuals without diabetes. Additionally, the study aims to identify factors that influence the polyphenol metabotype (transit time and gut microbial capacity to degrade polyphenols in particular).
Study Type
OBSERVATIONAL
Enrollment
100
Patients will receive a so-called oral polyphenol challenge test. It is a pill containing a multitude of polyphenol classes (doi: 10.1093/cdn/nzac072.020)
Fasting blood sample will be obtained for determination of markers of metabolic health.
Subjects collect morning urine (or the urine thereafter ) in a separate urine container and write down the time of urine collection for determination of polyphenol metabolites
Ghent University
Ghent, East Flanders, Belgium
NOT_YET_RECRUITINGDepartment of rehabilitation sciences - UGent
Ghent, Belgium
RECRUITINGDifferences in polyphenol metabolites in morning urine in persons with type 2 diabetes and in healthy controls.
The study aims to characterize the overall polyphenol metabotype in patients with T2DM compared to age-matched individuals without diabetes. For this purpose we will look at morning urine 24h after ingestion of a polyphenol rich pill.
Time frame: This will be assessed the morning after ingestion of the pill containing polyphenols
Differences in polyphenol metabolites in 24h urine in persons with type 2 diabetes and in healthy controls.
For this purpose participants will collect urine over a 24h period
Time frame: Urine will be collected over a 24h period after ingestion of the polyphenol pill.
Transit time
Along with the ingestion of the polyphenol pill, people will consume E133, a food colorant that will also color the stool.
Time frame: baseline
the capacity of the gut microbiome to degrade polyphenols
By using a batch experiment.
Time frame: baseline
Metabolic health
This will be determined on the fasting blood samples
Time frame: baseline
lifestyle factors and medical history (A)
physical activity, using the validated IPAQ questionnaire
Time frame: baseline
lifestyle factors and medical history (B)
Sleep, using the validated PSQI questionnaire
Time frame: baseline
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Subjects collect urine over the entire 24h. for determination of polyphenol metabolites
Questionnaires will be performed to collect information on lifestyle and baseline characteristics: physical activity (IPAQ), sleep (PSQI), drinking and smoking behaviour, drug intake and medical record.
Optional: some subjects collect stool samples to test the capacity of the microbiome to degrade polyphenols with a batch experiment.
Subjects take a pill containing 90mg of royal blue dye (E133) together with the polyphenol pill to determine their transit time. When their stool discolours, the subjects notify the researcher.