Certain populations, such as industrial workers and endurance athletes are particularly susceptible to dehydration due to exposure to heat for long periods of time causing evaporative water loss via sweating. The physical and cognitive decrements associated with dehydration have been widely researched, however the effect of dehydration on metabolism is lesser known. As climate change is causing temperatures to rise, and metabolic diseases such as type 2 diabetes are more common, the effect of dehydration on metabolism, specifically glycemic response to meals, needs to be established for future recommendations in clinical, environmental and athletic settings. Therefore, the aim of this study is to investigate the effect of acute exercise-induced dehydration on subsequent metabolic responses to feeding (i.e. glycaemia and insulinaemia).
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
16
Water ingested to fully replace sweat losses during exercise.
Water restriction to induce hypohydration of \~3% body mass through sweat losses during exercise.
Loughborough University
Loughborough, Leicestershire, United Kingdom
Plasma glucose
Measured using colorimetric assay from venous blood samples
Time frame: 6 hours
Plasma insulin
Measured using ELISA from venous blood samples
Time frame: 6 hours
Plasma osmolality
Measured using freezing point depression from venous blood samples
Time frame: 6 hours
Plasma volume change
Determined from haemoglobin and haematocrit measures in blood samples collected before and after drink ingestion
Time frame: 6 hours
Urine volume
Determined from urine samples collected before and after exercise
Time frame: 6 hours
Body mass
Determined from weighing participants before and after exercise
Time frame: 6 hours
Urine specific gravity/osmolality
Determined from urine samples collected before and after exercise
Time frame: 6 hours
Thirst sensation
Measured via 100 mm visual analogue scales 0 mm = not at all thirsty 100 mm = extremely thirsty
Time frame: 6 hours
Hunger sensation
Measured via 100 mm visual analogue scales 0 mm = not at all hungry 100 mm = extremely hungry
Time frame: 6 hours
Fullness sensation
Measured via 100 mm visual analogue scales 0 mm = not at all full 100 mm = extremely full
Time frame: 6 hours
Core body temperature
Measured via ingestible pill
Time frame: 6 hours
Blood pressure
Measured via and automated sphygmomanometer
Time frame: 6 hours
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.