Structural and Microbiological Characterization of Arterial Catheter Biofilm in ICU's Patients Using Optical Coherence Tomography

Centre Hospitalier William Morey - Chalon sur Saône60 enrolled

Overview

29.3% of bacteremias in intensive care units (ICU) are linked to vascular devices, including 7.7% to arterial catheters, with an influence on both morbility and mortality. It is now accepted that biofilm as a role on bacterial development on inner surface of vascular devices but there is yet a lack of clinical relevant data documenting a causal relation between biofilm formation and bacteremias. The investigators assume that a better structural and microbiological characterization of arterial catheter biofilm in ICU patients could help preventing bacteremias or have a more specific treatment when it appears.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Biofilms Arterial Catheterization, Peripheral Bacteremia Tomography, Optical Coherence

Interventions

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Patient or relative informed of the study and having declared their non-objection * Patient over 18 years old * Patient hospitalized in ICU with an arterial catheter Exclusion Criteria: * Patient unable to express consent * Patient whose collection of the arterial catheter is impossible or for whom storage at 4°C is impossible * Patient with arterial catheter removed outside the usual procedure of the ICU * Patient admitted in ICU with an arterial catheter already in place

Locations (2)

CHU Dijon Bourgogne

Dijon, Bourgogne-Franche-Comté, France

RECRUITING

CH Macon

Mâcon, Bourgogne-Franche-Comté, France

RECRUITING

Outcomes

Primary Outcomes

Biofilm structure type (ribbon-shaped or mushroom-shaped), measured with D-FF-OCT

Provide a better understanding of the relation between structural and microbiological characterization of artherial catheter biofilm in ICU patients using D-FF-OCT

Time frame: Outcome measure is assessed 2 days following catheter removal

Secondary Outcomes

Bacterial or fungal indentification

Identification and enumeration of species - whether bacterial or fungal - by mass spectrometry from culture in agar medium of biofilm samples

Time frame: Outcome measure is assessed 2 days following catheter removal

Biofilm thickness, measured with D-FF-OCT

Provide a better understanding of the relation between thickness and microbiological characterization of artherial catheter biofilm in ICU patients using D-FF-OCT

Time frame: Outcome measure is assessed 2 days following catheter removal

Biofilm dynamic signal distribution, measured with D-FF-OCT

Provide a better understanding of the relation between dynamic signal distribution and microbiological characterization of artherial catheter biofilm in ICU patients using D-FF-OCT

Time frame: Outcome measure is assessed 2 days following catheter removal

Structural and Microbiological Characterization of Arterial Catheter Biofilm in ICU's Patients Using Optical Coherence Tomography

Overview

Conditions

Interventions

Eligibility

Locations (2)

Outcomes

Primary Outcomes

Secondary Outcomes

Central Contacts