This prospective study aims to compare the effects of light and heavy paraffin oil in embryo culture during in vitro fertilization (IVF). Paraffin oil is commonly used in IVF laboratories to protect embryo culture media from evaporation and temperature fluctuations. However, the impact of different oil viscosities on embryo development and pregnancy outcomes remains unclear. Participants undergoing IVF treatment will have their embryos cultured under either light or heavy paraffin oil conditions. The study will evaluate key outcomes, including embryo development, blastocyst formation rates, and pregnancy rates. Findings from this research may help optimize embryo culture conditions, potentially improving IVF success rates and guiding best laboratory practices.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
TREATMENT
Masking
NONE
Enrollment
818
Embryos in this group will be cultured under conditions using light paraffin oil as an overlay in in vitro fertilization (IVF). The oil serves to protect the culture media from evaporation and temperature fluctuations. Key outcomes, including embryo development, blastocyst formation rate, and pregnancy rate, will be assessed.
Embryos in this group will be cultured under conditions using heavy paraffin oil as an overlay in in vitro fertilization (IVF). This study aims to compare the effects of heavy paraffin oil to light paraffin oil in embryo culture, assessing embryo development, blastocyst formation, and pregnancy rates.
Hung Vuong Hospital
Ho Chi Minh City, Vietnam
Fertilization Rate
The proportion of oocytes that successfully undergo fertilization after insemination, as determined by the presence of two pronuclei (2PN) at the appropriate time post-fertilization.
Time frame: 16-20 hours post-insemination
Cleavage Rate
The proportion of fertilized oocytes that progress to cleavage-stage embryos, assessed by the number of embryos that reach at least the two-cell stage within 48 hours after fertilization.
Time frame: 48 hours post-fertilization
Blastocyst Formation Rate
The proportion of embryos that successfully develop into blastocysts by Day 5 or Day 6 of culture, assessed based on morphological grading criteria.
Time frame: 5-6 days post-fertilization
Positive Beta-hCG
The proportion of participants achieving a positive serum beta-hCG test, indicating biochemical pregnancy, measured approximately two weeks after embryo transfer.
Time frame: 14 days post-embryo transfer
Implantation Rate
The proportion of transferred embryos that successfully implant in the uterus, determined by the presence of a gestational sac on ultrasound.
Time frame: 4-5 weeks post-embryo transfer
Clinical Pregnancy Rate
The proportion of participants with ultrasound-confirmed pregnancy, defined by the presence of a gestational sac with fetal heartbeat.
Time frame: 6-7 weeks post-embryo transfer
Ongoing Pregnancy Rate
The proportion of participants with a viable pregnancy continuing beyond the first trimester (≥12 weeks of gestation).
Time frame: 12 weeks post-embryo transfer
Live Birth Rate
The proportion of participants who achieve a live birth, defined as the delivery of a viable infant at ≥24 weeks of gestation.
Time frame: At delivery (up to ~40 weeks of gestation)
Pregnancy Loss Rate
The proportion of participants experiencing pregnancy loss at any stage
Time frame: From positive beta-hCG until delivery
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