Laboratory-based studies show that exposure to high humidity can worsen the effects of heat stress in young and older adults by impeding sweat evaporation - the main mechanism by which the human body cools itself. At high levels of humidity, the efficiency of sweating decreases causing a greater rise core temperature and burden on the cardiovascular system. In this context, increasing temperatures and humidity with climate change thus pose a potential compound risk for human health. While humidity's role in heat-health outcomes could substantially alter projections of health burdens from climate change, the impact of humidity on physiological strain in vulnerable people in relation to the indoor environment has yet to be evaluated. In a recent study delineating the physiological effects of the proposed 26°C indoor upper limit (PMID: 38329752), relative humidity was set to 45% in all conditions based on indoor humidity standards by the American Society of Heating and Air-Conditioning Engineers. However, it is unknown whether a refinement of the recommended indoor temperature limit of 26°C is required in situations where humidity cannot be maintained at this level. On separate occasions, the investigators will assess the change in body temperature and cardiovascular strain in older adults (65-85 years) exposed for 10 hours at the recommended indoor temperature limit of 26°C and 45% relative humidity (equivalent humidex of 29 (considered comfortable)) (experimental condition A), to 26°C with a relative humidity of 15% (equivalent humidex of 23 (considered comfortable); humidex is used to measure the perceived temperature taking into account the humidity)) (experimental condition B), to 26°C with a relative humidity of 85% (equivalent humidex of 37 (considered somewhat uncomfortable)) (experimental condition C), and to 31°C and 45% relative humidity with an equivalent humidex of 37 (considered somewhat uncomfortable) that is similar to experimental condition C. With this experimental design, investigators will assess the effects of indoor humidity in driving human heat strain and identify whether refinements in the recommended 26°C indoor temperature limit may be required. Further, by evaluating changes in relation to ambient conditions with a similar humidex, the investigators can assess how individuals perceive and respond to both heat and humidity.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
10
Older adults are exposed to a 10-hour simulated exposure
University of Ottawa
Ottawa, Ontario, Canada
RECRUITINGCore temperature (Peak) during daylong exposure to indoor overheating
Peak rectal temperature (15 min average) during exposure. Rectal temperature is measured continuously throughout the 10 hours of the simulated indoor overheating.
Time frame: End of 10 hour daylong exposure
Core temperature (AUC) during daylong exposure to indoor overheating
Areas under the curve (AUC) of rectal temperature during the 10 hour simulate indoor overheating.
Time frame: End of 10 hour daylong exposure
Heat rate (Peak) during daylong exposure to indoor overheating
Peak heart rate (15 min average) during exposure. Heart rate is measured continuously throughout the 10 hours of the simulated indoor overheating.
Time frame: End of 10 hour daylong exposure
Heart rate (AUC) during daylong exposure to indoor overheating
Areas under the curve (AUC) of heart rate during the 10 hour simulate indoor overheating.
Time frame: End of 10 hour daylong exposure
Cardiac response to standing from supine (30:15 Ratio) during daylong exposure to indoor overheating
Cardiac response to standing evaluated as the ratio between the highest R-wave to R-wave interval (lowest heart rate) measured at the 30th heart beat after standing from supine (+/- 5 beats) and the lowest R-wave to R-wave interval (highest heart rate) measured at the 15th heart beat after standing (+/- 5 beats). Cardiac response to standing will be evaluated twice, during two lying-to-standing tests (separated by 10 min of supine rest).
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Systolic Response to Standing From Supine during daylong exposure to indoor overheating
Systolic blood pressure response to standing evaluated as the difference in blood pressure measured between the standing and supine. Standing systolic blood pressure will be taken as the lowest value of those measured after 60 and 120 seconds of standing. Systolic response to standing will be evaluated twice, during two lying-to-standing tests (separated by 10 min of supine rest).
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Heart rate variability: RMSSD during daylong exposure to indoor overheating
Root mean squared standard deviation of normal-to-normal R-wave to R-wave intervals (RMSSD) measured during 5 minutes of paced breathing (15 breaths/min) with participants in the supine position. RMSSD will be evaluated twice, during two paced breathing periods (separated by 4 min of supine rest).
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Heart rate variability: SDNN during daylong exposure to indoor overheating
Standard deviation of normal-to-normal R-wave to R-wave intervals (SDNN) measured during 5 minutes of paced breathing (15 breaths/min) with participants in the supine position. SDNN will be evaluated twice, during two paced breathing periods (separated by 4 min of supine rest).
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Thermal comfort scale during daylong exposure to indoor overheating
Thermal comfort assessed via a visual analog scale ranging from extremely uncomfortable to extremely comfortable (midpoint: neutral).
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Thirst sensation scale during daylong exposure to indoor overheating
Thermal sensation assessed via a visual analog scale ranging from extremely hot to neutral (midpoint: hot).
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Arousal scale during daylong exposure to indoor overheating
Felt arousal scale assessed via a visual analog scale ("How worked up are you?") ranging from "high arousal" to "low arousal".
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Fluid loss during daylong exposure to indoor overheating
Average hourly fluid consumption calculated by weighing participant water intake at the start and end of each hour of exposure (normalized to the exposure duration).
Time frame: At end of 10 hour daylong exposure
Changes in plasma volume during daylong exposure to indoor overheating
Change in plasma volume from baseline values calculated from duplicate measurements of hemoglobin and hematocrit at the start and end of each exposure using the technique by Dill and Costill
Time frame: At end of 10 hour daylong exposur
Systolic blood pressure during daylong exposure to indoor overheating
Systolic blood pressure measured in triplicate via automated oscillometry (\~60 seconds between measures)
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Rate pressure product during daylong exposure to indoor overheating
Rate pressure product, an index of myocardial work and strain, calculated as systolic blood pressure x heart rate.
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Impulse control (cognitive function) during daylong exposure to indoor overheating
Participants will be provided with a tablet device with the Sway Medical testing platform for the assessment of impulse control. Participants will be asked to respond to both "go" and "no-go" visual cues. In response to the "go" cue, participants will initiate a movement of the device.
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Memory recall (cognitive function) during daylong exposure to indoor overheating
Participants will be provided with a tablet device with the Sway Medical testing platform for the assessment of memory recall. Participants complete both a delayed recall test and a working memory test.
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
CDC 4-Stage Balance Test (Postural stability) during daylong exposure to indoor overheating
To assess postural stability, participants hold a tablet to their chest, then auditory cues guide participants through four consecutive stances, feet side by side, instep of one foot touching the big toe of the other foot, tandem stand with one foot in front of the other, heel touching toe, and stand on one foot. The balance assessment will be evaluated based on movement detected by an accelerometer integrated into the hardware of the tablet device (Sway Medical Inc).
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
BTrackS Balance Assessment (Postural stability) during daylong exposure to indoor overheating
To assess postural stability, participants will be asked to stand on a BTracks force plate with their feet spread out to shoulder width, hand on their hips and eyes closed. The assessment will comprise of four trials (one practice trial) lasting 20 seconds in length and 20 seconds between trials. Center of pressure (COP) vector data along vertical (y) and horizontal (x) axes will be summed for the total path excursion length (cm) during each trial.
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Profiles of Mood States (POMS) during daylong exposure to indoor overheating
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Potential changes in mood (7 subscales of mood: tension, anger, depression, fatigue, confusion, vigor and esteem-related affect). The POMS-40 is a validated, self-administered questionnaire that examines seven distinct aspects of mood state across two positive subscales (Esteem-Related Affect, and Vigor) and five negative subscales (Fatigue, Tension, Confusion, Anger, and Depression), which are described across 40 distinct adjectives (reference). For each individual item, participants were asked to describe "how you feel right now" by responding using a 5-point Likert scale (0 = "Not at all", 1 = "A little", 2 = "Moderately", 3 = "Quite a lot", or 4 = "Extremely"). The values of items associated with a specific subscale (e.g., Fatigue) were summed to calculate its score.
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Environmental Symptoms Questionnaire (ESQ) during daylong exposure to indoor overheating
Self-reported environmental reactions and medical symptomatology associated with prolonged heat exposure. The ESQ-IV is a validated 68-item, self-administered questionnaire that has been used successfully in identifying symptomatology during exposure to a wide variety of environmental conditions, including heat exposure \[24, 25\]. Participants are asked to assess and described "how you have been feeling today" by responding to each item using a 6-point Likert scale (0 = "Not at all", 1 = "Slight", 2 = "Somewhat", 3 = "Moderate", 4 = "Quite a bit", or 5 = "Extreme"). Total Symptom Score was calculated from this data by taking the sum of the intensity ratings from all 68 individual items using reverse scores for the three positive items from the list ("I Felt Good", "I Felt Alert", and "I Felt Wide Awake").
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Sleepiness during daylong exposure to indoor overheating A
Karolinska sleepiness scale measures the subjective level of sleepiness at a particular time
Time frame: At the start (hour 0) and end of 10 hour daylong exposure
Sleepiness during daylong exposure to indoor overheating B
Stanford sleepiness scale consists of only one item, where participant must identify one of seven statements that best represents their level of perceived sleepiness. It employs a scale of 1 to 7 with 1 = feeling wide awake and 7 = sleep onset soon
Time frame: At the start (hour 0) and end of 10 hour daylong exposure