Intense physical exercise can lead to micro-damage to tissues and activation of inflammatory processes, the body's natural response to exercise-related metabolic stress. Monitoring inflammation and fatigue will enable the observation of athletes in terms of post-exercise regeneration and muscle adaptation to physical effort. The obtained results will allow for estimating the level of fatigue, minimizing the risk of injury, and will show the rate of post-exercise regeneration or its absence.
The study will involve 40 members of the Polish Youth Rowing Team, both sexes, aged 19-23. The study will be held on three dates related to the specifics of the athletes' training cycle, i.e. in the preparatory period (March), the competition period (June), and the detraining period (November). The participants will perform an intensive exercise test on a rowing ergometer (manufacturer: Concept II, USA), covering a distance of 2,000 meters in the shortest possible time. The study will involve 40 members of the Polish Youth Rowing Team, both sexes, aged 19-23. Each time, before starting the exercise test, a non-invasive anthropometric analysis will be performed using the TANITA MC-780MA body composition analyzer (tanita Japan), to determine the body composition: percentage of body fat, body fat mass, lean tissue mass, muscle mass, body water and body mass index (BMI). On the three indicated dates, before and after the stress test, capillary blood (ear lobe) from the cubital vein will be collected from the participants. Using immunoenzymatic ELISA tests, the following will be determined: EPO (erythropoietin), VEGF (vascular endothelial growth factor), CRP (C-reactive protein), glycogen, hypoxanthine (Hx). Glucose level will be determined using an Accu-Chek Active glucometer (Roche, Germany).
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
OTHER
Masking
NONE
Enrollment
40
The examined rowers will be subjected to an effort on a rowing ergometer, consisting of covering a designated distance in the shortest possible time.
Poznań University of Physical Education, Faculty of Physical Culture in Gorzów Wielkopolski
Gorzów Wielkopolski, Polska, Poland
EPO (erythropoietin) level
Immunoenzymatic assay method using a diagnostic ELISA Kit
Time frame: Immediately before the ergometer test, immediately after and 3 hours after the test
VEGF (vascular endothelial growth factor) level
Immunoenzymatic assay method using a diagnostic ELISA Kit
Time frame: Immediately before the ergometer test, immediately after and 3 hours after the test
Hypoxanthine (Hx) level
Immunoenzymatic assay method using a diagnostic ELISA Kit
Time frame: Immediately before the ergometer test, immediately after and 3 hours after the test
Glycogen level
Immunoenzymatic assay method using a diagnostic ELISA Kit
Time frame: Immediately before the ergometer test, immediately after and 3 hours after the test
Glucose level
Using an Accu-Chek Active glucometer (Roche, Germany)
Time frame: Immediately before the ergometer test, immediately after and 3 hours after the test
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