Hormone Replacement Therapy in Adolescents With Premature Ovarian Insufficiency

Overview

Background: Premature ovarian insufficiency (POI) is a condition in which women under the age of 40 years have absent or irregular menstrual cycles. POI can cause infertility, signs of menopause, osteoporosis, and other symptoms. Hormone replacement therapy (HRT) is a treatment that gives women extra hormones, such as estrogen and progesterone. HRT works well in adult women. Researchers want to find the most effective doses and regimens for adolescents. Objective: To monitor the effects of HRT on adolescents with POI. Eligibility: Female adolescents aged 11 to 19 years diagnosed with POI. Healthy volunteers are also needed. Design: All participants will have clinic visits every 6 months for 2 years. Each visit may last 2 days. Each visit may include: Blood and urine tests. A test of their heart function. A test to measure the stiffness of their blood vessels. Participants will lie flat with a blood pressure cuff on a leg and a meter on the neck while the cuff inflates. A test of their grip strength. Participants will squeeze a handheld device as hard as they can. Two scans to measure bone density. For one, participants will lie on a table while a scanner passes along their body. For the other, participants will sit in a chair and insert their forearm, then their lower leg, into a scanner. A test to measure skin pigmentation. Participants' skin will be touched lightly with a device. An optional visual exam of the vagina. Some vaginal fluid may also be collected with a cotton swab/cytobrush. Participants with POI will receive HRT. They will be given estrogen patches and progesterone pills.

Study Description: This is a study of adolescents with premature ovarian insufficiency (POI) and their response to hormone replacement therapy (HRT). Objectives: Primary: To identify the phenotype and associated genotype of adolescents with POI at the time of diagnosis, including assessment of overall bone health of participants pre- and post-HRT treatment with respect to bone mineral density (BMD) and other skeletal endpoints. Bone Health: -Follow patients with POI for bone health assessments: * Measure BMD at different skeletal sites, including the central skeleton (lumbar spine, total body less head, and hip - femoral neck and total hip) * Perform vertebral fracture assessment (VFA) * Determine body composition of lean body and fat mass (total body) and correlate with BMD. * Assess skin pigmentation and muscle grip strength and correlate with BMD Secondary: Bone Health -Follow patients with POI for bone health assessments: * Measure BMD at peripheral skeleton (radius and tibia) * Evaluate bone microarchitecture and strength of the radius and tibia. Metabolic Phenotype: * Follow patients with POI for metabolic assessment: * Insulin resistance * Cardiovascular risk * Lipid profiles * Bone turnover Muscle Phenotype: -Follow patients with POI for muscle function assessment: * Determine hand grip muscle strength * Evaluate lean body mass (by DXA) to determine muscle mass Cardiovascular Health -Follow patients with POI for a cardiovascular evaluation: * Electrophysiologic parameters (from 12-lead EKG) * Arterial stiffness (by Pulse Wave Velocity, PWV) * Coagulation studies to determine the impact of HRT on clotting function Genetic profiling: -If obtained, assess the baseline genotype of patients with POI to correlate with BMD and other health outcomes --If subjects are co-enrolled on NIAID protocol 17I0122, utilize that genetic sequencing data to associate with genotype and response of multiple health outcomes to HRT. Quality of Life: -Follow patients with POI for evaluation of an individual s perception of their life quality and health: * Evaluate the individual s perceived occupational performance in self-care, productivity, and leisure * Evaluate the individual s occupations or hobbies that align with their interests Psychological health: -Follow patients with POI for evaluation of an individual s mood and well-being: * Measure symptoms and severity of depression * Measure the state and trait components of anxiety Menopause profile: -Follow patients with POI for determining signs and symptoms of menopause: * Evaluate reproductive hormonal changes * Assess menopausal symptoms and their severity * Evaluate vaginal health Hormone Replacement Therapy: -Follow patients with POI longitudinally to determine the most effective HRT management: * Evaluate dosage, delivery, combination of HRT * Evaluate effects of HRT on bone, cardiovascular, metabolic, gynecologic, and psychological health Endpoints: Primary: Longitudinal evaluation of BMD of the central skeleton (lumbar spine). As secondary measures of bone health, dual-energy x-ray absorptiometry (DXA) measures of skeletal sites other than the spine (DXA - hip/total body) will be evaluated longitudinally in POI participants and their BMD compared to healthy control participants. Using DXA, vertebral fractures assessment (VFA) will be performed in POI participants and compared to healthy control participants. In addition, BMD will be evaluated with respect to skin pigmentation and muscle grip strength. Secondary: * High-resolution peripheral quantitative computed tomography (HRpQCT) bone density and strength measures by HRpQCT of the peripheral skeleton (radius and tibia) will be evaluated longitudinally in participants with POI and their BMD compared to healthy control participants. * Compare metabolic and cardiovascular parameters in participants with POI to healthy controls. * Compare muscle phenotype in participants with POI to healthy controls by measuring muscle strength using hand grip dynamometry and measuring lean body mass (as measure of total body muscle mass) by DXA. * Compare repolarization changes (by 12-lead EKG), arterial stiffness (by PWV) and coagulation parameters in participants with POI to healthy controls. * Compare genetic background of participants with POI to healthy controls using whole genome sequencing. * Evaluate quality of life with Canadian Occupational Performance Measure (COPM) and Interest Checklist questionnaires. * Evaluate psychological health of participants with POI compared to healthy controls using the Beck Depression Inventory and Spielberger State/Trait Anxiety Inventory. * Evaluate menopausal profile in participants with POI compared to healthy controls by detecting changes in ovarian hormonal levels and correlating with responses on the Menopause Rating Scale, and vaginal cytology and degree of atrophy after HRT compared to baseline (and participants vs. controls). * Compare health effects from a typical clinical HRT regiment over the course of 2 years of administration.