The main research hypothesis is that alterations in the communication between the endoplasmic reticulum (ER) and the mitochondria at contact sites called mitochondria-associated membranes (MAMs) occurs in different hepatic cell types of patients with Metabolic Dysfunction-Associated Steatotic Liver Disease (MALSD) and is involved in the progression towards MASH and could also influence the process of improvement of MASH. This study aims to investigate the link between Metabolic Dysfunction-Associated Steatohepatitis (MASH) and Mitochondria-Associated Membranes (MAMs) in liver cells and peripheral blood mononuclear cells (PBMCs) in patients undergoing bariatric surgery. The primary objective is to analyze MAMs alterations in hepatocytes in MASH patients compared to non-MASH patients. Secondary objectives include evaluating the correlation between MAMs in PBMCs and liver cells and assessing MAMs changes post-bariatric surgery.
Study Type
OBSERVATIONAL
Enrollment
20
Patients undergoin bariatric surgery with simultaneous liver biopsy to analyze MAMs alterations in liver and PBMCs. Follow-up assessments at 6 and 12 months.
Hospices Civils de Lyon - Hôpital Edouard Herriot
Lyon, France, France
RECRUITINGCentre Hospitalier Lyon Sud, Endocrinologie, Diabète et nutrition
Pierre-Bénite, France, France
RECRUITINGMAMs alterations in hepatocytes
Number of MAM contacts in liver cells at baseline (T0)
Time frame: Baseline (T0)
MAMs alterations in non-parenchymal liver cells (T0)
Number of MAM contacts in non-parenchymal liver cells at baseline (T0)
Time frame: Baseline (T0)
MAMs alterations in PBMCs (T0, T6, T12 months)
Number of MAM contacts in PBMCs
Time frame: Baseline (T0) , T6, T12 months
Changes in hepatic markers of MASLD post-surgery
Changes in measurements, of Vibration Controlled Transient Elastographt (VCTE) after bariatric surgery (∆T0-T6months, ∆T6-T12months and ∆T0-T12months).
Time frame: T0 to T6, T6 to T12, T0 toT12 (months)
Changes in hepatic markers of MASLD post-surgery (2)
Controlled attenuation Parameter (CAP), after bariatric surgery (∆T0-T6months, ∆T6-T12months and ∆T0-T12months).
Time frame: T0 to T6, T6 to T12, T0 toT12 (months)
Correlation between MASH improvement and MAMs changes
Correlation between MASH improvement and MAMs changes
Time frame: T0-T12 months
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