Effect of Acute Protein Supplementation in Diabetic Patients

University of Malaya20 enrolled

Overview

The goal of this trial is to test the effect of house cricket (Acheta domesticus) preload on glycemic response in diabetic patients. The main question\[s\] it aims to answer are: whether house cricket powder could lower blood glucose spike and stimulate higher insulin response. Participants will consume either house cricket powder, whey protein isolate or placebo which will be mixed with a chicken-flavored soup. Participants fingers will be pricked for up to 2 hours. Researcher will compare house cricket powder with placebo to see if the glycemic response is better in house cricket supplemented group. Researcher will compare house cricket powder with whey protein isolate to see if both supplement induce comparable glycemic response.

Participants blood will be collected at 8 time points: Baseline 20 minutes after pre load 15 minutes after Oral Glucose Tolerance Test (OGTT) 30 minutes after OGTT 45 minutes after OGTT 60 minutes after OGTT 90 minutes after OGTT 120 minutes after OGTT

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

PREVENTION

Masking

TRIPLE

Enrollment

Conditions

Diabetes Mellitus Type 2

Interventions

Eligibility

Sex: MALE

Medical Language ↔ Plain English

Inclusion Criteria: * Suffering from type 2 diabetes mellitus (HbA1c \> 6.5%) * Consume metformin for at least 6 months Exclusion Criteria: * Consume other type of anti-diabetic drugs * Presenting any food allergy * Recent surgery or infections that may affect oral glucose tolerance test * Consume other drugs that may affect oral glucose tolerance test * Not consuming any anti-diabetic drugs * Pre-diabetes (HbA1c 5.7 - 6.4%)

Outcomes

Primary Outcomes

Blood glucose

Blood glucose concentration measured using glucometer. Participants fingers are pricked and using capillary tube, a drop of the blood is dripped in a test strip before inserted to a glucometer.

Time frame: 120 minutes

Secondary Outcomes

Blood insulin

Blood sample is collected through a finger prick. The blood will be centrifuged to obtain the serum. Insulin serum concentration is then measured by using ELISA kits. The blood sample are treated following the kit guideline, and the insulin concentration are measured by comparing the light absorbance against a prepared standard.