The influence of a 6000m ergometer test on the markers of gut injury, endotoxemia, iron status, stress and inflammation in elite rowers.
The principal objective of our study was to assess gut permeability after completing a 6000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 6000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1 hour of restitution. Parameters, such as I-FABP (intestinal fatty acid binding protein), LPS (lipopolysaccharide), LBP (lipopolysaccharide binding protein), claudin3, ferritin, transferrin, hepcidin, IL-6 (Interleukin-6), HO-1 (Heme Oxygenase 1), and ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) were determined using appropriate biochemical tests.
Study Type
OBSERVATIONAL
Enrollment
21
Participant perforemed 6000m ergometer test.
Poznan University of Physical Education
Gorzów Wielkopolski, Lubusz Voivodeship, Poland
I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury
I-FABP concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LBP (lipopolysaccharide binding protein) to measure endotoxin
LBP concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Claudin 3 to measure tight junction leakage
claudin 3 concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LPS (lipopolysaccharide) to measure endotoxin
LPS concentration measured in blood \[EU/Ll\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Lactic acid to measure fatigue after the race
Lactate concentration measured in capillary blood from earlobe \[mmol/L\] using a commercially available kit (Diaglobal, Berlin, Germany)
Time frame: Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.
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Ferritin to measure iron status
Ferritin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
Transferrin to measure iron status
Transferrin concentration measured in blood \[mg/ml\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
Hepcidin to measure iron status
Hepcidin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
IL-6 (Interleukin-6) to measure inflammation
IL-6 concentration measured in blood \[ng/l\] using commercially available enzyme-linked immunosorbent assays.
Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
HO-1 (Heme Oxygenase 1) to measure oxidative stress
HO-1 concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) to measure oxidative stress
ACSL4 concentration measured in blood \[pg/ml\] using commercially available enzyme-linked immunosorbent assays.
Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
energy
energy \[kcal \]of food intake measured by 24h dietary recall
Time frame: day before test, in the morning before the test
protein
protein \[g \]of food intake measured by 24h dietary recall
Time frame: day before test, in the morning before the test
carbohydrate
carbohydrate \[g \]of food intake measured by 24h dietary recall
Time frame: day before test, in the morning before the test
fat
fat \[g \]of food intake measured by 24h dietary recall
Time frame: day before test, in the morning before the test
fiber
fiber \[g \]of food intake measured by 24h dietary recall
Time frame: day before test, in the morning before the test
body mass
Body mass \[kg\] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Time frame: in the morning before the test
Body composition - water
% of body water measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Time frame: in the morning before the test
Body composition - fat
% of body fat measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Time frame: in the morning before the test
Lean body mass
Lean body mass \[kg\] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Time frame: in the morning before the test
Height
Prior to the exercise test, we measured the anthropometric parameters, including height (Seca 213 Hamburg, Deutschland) \[cm\]
Time frame: in the morning before the test
Gastrointestinal sypthomps self reported scale
10 point scale were 10 is a outcome of the worst severity
Time frame: up to 1 hours after the test