Tracing Of Real-time glu13Cose Metabolism in Human Immune Cells

Vanderbilt University Medical Center12 enrolled

Overview

The purpose of this study is to understand how cells of the immune system use the common sugar glucose to fuel energy production and as a building block within the cell. Investigators will intravenously infuse a non-radioactive glucose tracer into participants over a few hours and collect immune cells from the blood to track uptake and usage of this glucose within these immune cells.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Glucose Metabolism Isotope Labeling Immune System and Related Disorders

Interventions

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Adults aged 18 or older Exclusion Criteria: * Pregnant * Prisoner or in police custody * Uncontrolled hyperglycemia/diabetes * Current participation in another study where the total volume of blood drawn, when added to this study blood draw volume, exceeds 500cc in an 8 week period * Any medical issue or pharmacologic exposure which, in the opinion of the study investigator, that might interfere with the study objectives * Any reason which, in the opinion of the study investigator, adds additional risk to the participant

Outcomes

Primary Outcomes

13C6-glucose uptake in immune cells

Detection of 13C6-glucose-derived metabolites in neutrophils, monocytes, CD4 T cells, and CD8 T cells using gas and liquid chromatography-mass spectrometry (GC-MS and LC-MS) following magnetic enrichment of these immune cell subsets.

Time frame: Up to 4 hours