Evaluation of the Potential Effects of LRP5 and Tph1 in the Pathogenesis of Periodontal Disease Individuals With Stage III Grade B Periodontitis

Ankara University40 enrolled

Overview

Objectives: The aim of this study was to determine the changes in LRP5 and TPH1 levels in serum and saliva samples due to periodontal inflammation and to evaluate the relationship between these values and clinical periodontal parameters. Methods: Saliva and serum samples were collected from 20 systemically healthy patients with Stage III periodontitis and 20 periodontally healthy control individuals. Salivary and serum LRP5 and TPH1 levels were analyzed using enzyme-linked immunosorbent assay (ELISA). Clinical periodontal parameters, including plaque index (PI), probing pocket depth (PPD), bleeding on probing (BOP), and clinical attachment level (CAL), were recorded.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Periodontal Disease Periodontitis (Stage 3)Periodontitis Stage III

Eligibility

Sex: ALLMin age: 18 YearsMax age: 70 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * \>18 years old, * At least 16 permanent teeth except 3rd molars, * Individuals who do not use orthodontic appliances, * Individuals who are not pregnant or lactating, * Individuals without any systemic disease that may affect periodontal health, * Systemically healthy individuals, * Individuals who have not used anti-inflammatory and/or anti-microbial drugs in the last 6 months, * Individuals who have not received periodontal treatment in the last 1 year Exclusion Criteria: * Pregnant/lactating individuals, * Individuals who have used anti-inflammatory and/or anti-microbial drugs in the last 6 months, * Individuals who have undergone periodontal treatment in the last 1 year, * Individuals with psychiatric illness, * Individuals with any oral infection, * Individuals with \<16 teeth, excluding molars, * Individuals with alcohol dependence, * Individuals with active infectious disease (acute hepatitis, AIDS, tuberculosis), cancer or any systemic condition that may affect periodontal tissues, * Individuals receiving treatment with drugs known to affect periodontal tissues (phenytoin, cyclosporine A, calcium channel blockers)

Outcomes

Primary Outcomes

LRP5

Low-density lipoprotein (LDL) receptor-related protein 5 (LRP5) is a WNT coreceptor in the LRP superfamily involved in a wide range of biological processes including endocytosis, cellular communication, lipid homeostasis and embryonic development. The levels of LRP5 in both serum and saliva samples were determined using commercially available ELISA kits. All assays were conducted according to the manufacturers' instructions.

Time frame: 1 month

TPH1

The inhibitory, irreversible enzyme of serotonin synthesis from intestinal cells is tryptophan hydroxylase 1 (TPH1). The levels of TPH1 in both serum and saliva samples were determined using commercially available ELISA kits. All assays were conducted according to the manufacturers' instructions.