This study is a randomized, double-blind, placebo-controlled trial designed to examine the effects of pre-sleep creatine monohydrate supplementation on anaerobic performance and muscle damage markers in recreationally active females. Participants will be randomly assigned to receive either 5 grams of creatine monohydrate or a placebo 30 minutes before sleep for seven consecutive days. Anaerobic performance will be assessed using the Wingate Anaerobic Test, evaluating peak and average power output and fatigue index. Blood samples will be collected before and after the supplementation period to analyze markers of muscle damage, including creatine kinase and lactate dehydrogenase. This study aims to provide insight into the efficacy of short-term, time-specific creatine supplementation for enhancing performance and recovery.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
NONE
Enrollment
24
Participants will receive 5 grams of creatine monohydrate powder dissolved in water, flavored with a sugar-free agent, 30 minutes before sleep each night for 7 days.
Visually identical placebo (maltodextrin powder, 5 g/day), flavored with a sugar-free agent, dissolved in water and consumed 30 minutes before sleep for 7 consecutive days.
Islamic Azad University, Shabestar Branch
Shabestar, East Azerbaijan Province, Iran
RECRUITINGAverage Power Output (Watts) - 30-Second Wingate Test
Average anaerobic power output will be calculated across the 30-second Wingate cycling test.
Time frame: Baseline and post-intervention (Day 0 and Day 7)
Peak Power Output (Watts) - Wingate Test
Peak power output during the initial 5 seconds of the 30-second Wingate cycling test.
Time frame: Baseline and post-intervention (Day 0 and Day 7)
Fatigue Index (%) - Wingate Test
Calculated as the percent drop in power from peak to minimum power output during the 30-second Wingate test, indicating fatigue rate.
Time frame: Baseline and post-intervention (Day 0 and Day 7)
Serum Creatine Kinase (CK) Activity
Serum creatine kinase activity will be measured as a marker of muscle damage using a spectrophotometric assay.
Time frame: Baseline and 24 hours post Wingate Test (Day 0 and Day 8)
Serum Lactate Dehydrogenase (LDH) Activity
Serum lactate dehydrogenase activity will be measured as an indicator of cell membrane permeability and muscle damage using spectrophotometry.
Time frame: Baseline and 24 hours post Wingate Test (Day 0 and Day 8)
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