This study will be aimed to investigated the correlation between levels of salivary amino acids (glycine , proline , lysine , alanine ) in the saliva of healthy children with and without active carious lesions and to estimate the predictive capacity of caries susceptibility
80 healthy children (8-10 years old) will be recruited from primary schools of Baghdad city/ Iraq, will be classified according to their level of caries activity (high caries activity, n = 40; negative caries activity, n = 40). Total stimulated and unstimulated saliva will be collected to estimate the caries activity by using the Snyder test, and Amino acid analysis of the saliva will be performed by reversed phase - high performance liquid chromatography (RP-HPLC), respectively.
Study Type
OBSERVATIONAL
Enrollment
80
The investigators will measure Salivary-free amino acids by using reverse phase high performance liquid chromatography
Salivary-free amino acid analysis
Unstimulated saliva collection for salivary free amino acids analysis
Time frame: Baseline :unstimulated saliva collection for salivary glycine , proline , lysine and alanine measurement
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