The study aims to investigate the acute effects of different protein sources (i.e., casein, whey, soy) on metabolomic profiles (e.g., amino acids in the blood), along with other outcomes such as cognitive function, mood, and appetite. While the study does not directly aim to prevent, prognosticate, diagnose, or alleviate any disease, it may provide insights that could inform dietary recommendations and support disease prevention efforts in older adults.
This study will be a double-blind, randomised, placebo-controlled trial using a crossover study design. 25 older adults (aged 60 to 80) will be recruited and assigned to consume 4 different test drinks in a random order on 4 different visits. This study aims to investigate whether higher protein consumption of different sources will influence postprandial plasma and urinary metabolomic profiles, cognition, mood and appetite. The study will comprise 1 screening visit and 4 test visits separated separated by a minimum washout period of \>7 days
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
TRIPLE
Enrollment
32
30 g protein equivalent of casein protein isolate dissolved in \~250 mL of water
30 g protein equivalent of whey protein isolate dissolved in \~250 mL of water
30 g protein equivalent of soy protein isolate dissolved in \~250 mL of water
NUS, Department of Food Science and Technology, 1 Science Drive 3, Block S13, #03-07
Singapore, Singapore
Changes in metabolites found in plasma (plasma metabolomics)
Untargeted metabolomics profiling of plasma will be conducted using ultra performance liquid chromatography-quadrupole time of flight-mass spectrometry (UPLC-QTOF-MS)
Time frame: Baseline, 15, 30, 60, 90, 120, 180, 240, 300 minutes
Changes in metabolites found in urine (urinary metabolomics)
Untargeted metabolomics profiling of urine will be conducted using ultra performance liquid chromatography-quadrupole time of flight-mass spectrometry (UPLC-QTOF-MS)
Time frame: Baseline, 60, 120, 180, 240, 300 minutes
Cognitive Function
The Computerised Mental Performance Assessment System (COMPASS) will be administered via a laptop. The COMPASS tests consist of 5 consecutive tests, including Digit Vigilance, Stroop, Rapid Visual Information Processing, Computerised Corsi Blocks Lite, Alphabetic Working Memory and Serial Subtraction, where the correctness and reaction time will be recorded. The test is expected to take around 15 minutes.
Time frame: Baseline, 15, 60, 120, 180, 240, 300 minutes
Mood
The Profile of Mood States (POMS) questionnaire will be administered, which contains various words that describe feelings. Participants will indicate, for each word, how they currently feel on scale of 0 (Not at all) to 4 (Extremely).
Time frame: Baseline, 15, 60, 120, 180, 240, 300 minutes
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30 g carbohydrate equivalent of maltodextrin powder dissolved in \~250 mL of water