The goal of this clinical trial is to characterize blood vessel function and oxidative stress (a harmful condition that damages cells and tissues) in emergency medical technicians (EMTs). The main questions it aims to answer are: 1. Does an overnight shift work in emergency medical technicians reduce blood vessel function and increase oxidative stress? 2. Can supplementing with antioxidants help reduce the negative effects of night shift work in emergency medical technicians? Researchers will compare antioxidants to a placebo (a look-alike substance that contains no drug) to see if antioxidants work in reducing the negative effects of night shift work in emergency medical technicians. Participants will: 1\. Report to the lab two separate times following an overnight shift to assess blood vessel functioning and oxidative stress 3. Take an antioxidant supplement or placebo during each night of shift work.
Heart disease, also referred to as cardiovascular disease, is the leading cause of death worldwide, responsible for about 30% of all deaths. While eating right, staying active, and avoiding cigarettes are key to preventing heart problems, the type of work a person does matters too. Some jobs can negatively affect heart health, especially those with night or irregular shifts. Emergency medical technicians (EMTs), the first responders who help keep our communities safe, often work these challenging schedules. Shift work means working outside normal business hours-like evenings, nights, and weekends. These irregular schedules, especially overnight shifts, can disrupt the body's natural sleep cycle. This disruption can cause health problems because it goes against the body's internal clock, or circadian rhythm. This rhythm controls important functions like sleep, hormone levels, and heart health. When someone works at night, this clock is disrupted, which can lead to higher stress levels, worse blood vessel function, and even long-term damage to the heart. Research shows that people who work night shifts have a higher risk of heart disease compared to those who work during the day. The risk increases even more the longer someone works night shifts. In fact, studies show that each additional five years of night shift work raises the risk of heart problems by 7%. One night of shift work is all it takes to cause damage to blood vessels and spike stress levels in the body. This study will examine how night shift work affects emergency medical technicians' heart and blood vessel health. The investigators will compare the function of their blood vessels and levels of oxidative stress (a harmful condition that damages cells and tissues) after working two overnight shifts. To understand how to best help reduce the negative effects of night shift work, this study will also test whether taking antioxidants-substances that protect the body from damage induced by oxidative stress-during one of the shifts can improve blood vessel health and reduce oxidative stress. Emergency medical technicians are a group that have not been researched much in this area and by doing so, this study hopes to learn more about how night shift work affects heart and blood vessel health. If antioxidants can help, this could lead to new ways of protecting shift workers from long-term heart problems-especially emergency medical technicians and first responders, who are vital to our community's safety.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
SINGLE
Enrollment
45
Participants will take two doses of antioxidant supplements in capsule form during their overnight shift: the first dose at 7:00 PM, and the second dose 1.5 hour before their morning vascular function visit (post-shift). Each dose will consist of the following concentrations of antioxidants: 600 mg of α-lipoic acid, 1,000 mg of vitamin C, and 600 IU of vitamin E.
Participants will take two doses of antioxidant supplements in capsule form during their overnight shift: the first dose at 7:00 PM, and the second dose 1.5 hour before their morning vascular function visit (post-shift). Each dose will consist of placebo pills made from microcrystalline cellulose, designed to match the antioxidants in taste, color, and appearance.
University of Delaware
Newark, Delaware, United States
RECRUITINGFlow Mediated Dilation (FMD)
Flow-mediated dilation (FMD) is a frequently practiced non-invasive technique which images artery structure and blood flow before, during, and after a period of blood flow occlusion and subsequent increases in blood flow. This creates stress on the inner lining (i.e., endothelium) of the vasculature, inducing a cascade of events which results in relaxation of the smooth muscle surrounding the vessel. Reduced functioning of this mechanism is significantly associated with cardiovascular complications and disease. FMD will be used to assess the diameter and blood flow velocity of the artery located in the upper arm via ultrasonography.
Time frame: Visit 2 (1 day)
Oxidative Stress
Electron Paramagnetic Resonance (EPR) Spectroscopy measurements will be conducted using a specialized spectrometer (Bruker EMX Nano) which is designed to detect unpaired electrons in biological samples, a key indicator of oxidative damage. For the EPR procedure, frozen samples of blood plasma will be carefully collected from the participant following standard clinical protocols during their vascular function visits. Briefly, the spin trapping technique will be used where a stabilizing molecule (e.g., spin probe such as cyclic hydroxylamine \[CMH\]) reacts with the free radical (e.g., superoxide) to form a more stable radical species for analysis (nitroxide). The stable sample will then be placed in the EPR resonator, and the spectrometer will be calibrated using known standards to ensure accurate detection of nitroxide.
Time frame: Visit 3 (1 day)
Passive Leg Movement (PLM)
PLM is a newer, non-invasive method of assessing vascular function that is also mediated through the responsiveness of the endothelium. This assessment may provide additional insight on vascular function specific to the lower extremity. Briefly, participants will have one minute of blood flow velocity of the right femoral artery recorded (distal to inguinal crease, proximal to deep and superficial bifurcation) via Doppler ultrasound, while sitting with back upright, both legs supported, and knees fully extended (i.e., 180 degrees). Passive movement will then be initiated by a second team member who will continuously move the participant's right leg through 90 degrees of motion for two minutes. Participants will be urged to remain passive and to avoid any assistance with limb motion. Blood flow velocity of the right femoral artery will be continuously recorded during the entire 3 minute test to investigate changes in blood flow velocity during passive leg movement.
Time frame: Visit 2 (1 day)
Flow Mediated Dilation (FMD)
Flow-mediated dilation (FMD) is a frequently practiced non-invasive technique which images artery structure and blood flow before, during, and after a period of blood flow occlusion and subsequent increases in blood flow. This creates stress on the inner lining (i.e., endothelium) of the vasculature, inducing a cascade of events which results in relaxation of the smooth muscle surrounding the vessel. Reduced functioning of this mechanism is significantly associated with cardiovascular complications and disease. FMD will be used to assess the diameter and blood flow velocity of the artery located in the upper arm via ultrasonography.
Time frame: Visit 3 (1 day)
Passive Leg Movement (PLM)
PLM is a newer, non-invasive method of assessing vascular function that is also mediated through the responsiveness of the endothelium. This assessment may provide additional insight on vascular function specific to the lower extremity. Briefly, participants will have one minute of blood flow velocity of the right femoral artery recorded (distal to inguinal crease, proximal to deep and superficial bifurcation) via Doppler ultrasound, while sitting with back upright, both legs supported, and knees fully extended (i.e., 180 degrees). Passive movement will then be initiated by a second team member who will continuously move the participant's right leg through 90 degrees of motion for two minutes. Participants will be urged to remain passive and to avoid any assistance with limb motion. Blood flow velocity of the right femoral artery will be continuously recorded during the entire 3 minute test to investigate changes in blood flow velocity during passive leg movement.
Time frame: Visit 3 (1 day)
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.